ArticleNature biomedical engineering2023
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy.
Article in Nature biomedical engineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
80 citing papers in PubMed, 164 citations in OpenAlex.
- Parallel multilayer photoacoustic microscopy of slide-free histology using a droplet-shaped UV beam.Science advances · 2026Article
- Label-free ultraviolet photoacoustic microscopy for multiscale structural phenotyping of epithelial morphogenesis in organoid-derived intestinal epithelial wrinkle tissue.Photoacoustics · 2026Article
- A Label-Free Ultraviolet Photoacoustic Microscopy Enables Nuclear Imaging and Toxicity Assessment in an Intact Brain Organoid.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Hardware-Attentive Programmable Fourier Ptychography Enables Task-Adaptive Label-Free Virtual Staining.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Virtual Overlay Staining With Plasmonic Oligomer Metasurface.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Ultraviolet Sensing-Guided Biomedical Systems: From Label-Free Imaging to Dosimetry and Therapy Feedback.Biosensors · 2026Review
- Photon absorption remote sensing (PARS): comprehensive absorption imaging enabling label-free biomolecule characterization and mapping.Scientific reports · 2026Article
- Multifocal Optical-Resolution Photoacoustic Microscopy With a Masked Single-Element Transducer.IEEE transactions on medical imaging · 2026Article
- Label-free pathological subtyping of non-small cell lung cancer using deep classification and virtual immunohistochemical staining.NPJ digital medicine · 2026Article
- Label-free whole slide virtual multi-staining using dual-excitation photon absorption remote sensing microscopy.Npj imaging · 2026Article
- Single capture quantitative oblique back-illumination microscopy.Npj imaging · 2026Article
- Photoacoustic Microscopy for Multiscale Biological System Visualization and Clinical Translation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- FPM2Stain Net: physics-guided super-resolution and multi-modal virtual staining for digital histopathology.Biomedical optics express · 2026Article
- Rapid Arbitrary-Shape Microscopy of Unsectioned Tissues for Precise Intraoperative Tumor Margin Assessment.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Deep Learning-Enabled Virtual Multiplexed Immunostaining of Label-Free Tissue for Vascular Invasion Assessment.BME frontiers · 2026Article
- A robust and scalable framework for hallucination detection in virtual tissue staining and digital pathology.Nature biomedical engineering · 2025Article
- Reduction of photobleaching effects in photoacoustic imaging using noise agnostic, platform-flexible deep-learning methods.Journal of biomedical optics · 2025Article
- Rapid cancer diagnosis using deep learning-powered label-free subcellular-resolution photoacoustic histology.Science advances · 2025Article
- Deep UV laser scanning microscopy with integrated 1050 nm spectral-domain optical coherence tomography for multi-contrast tissue imaging.Biomedical optics express · 2025Article
- The Evolution of Machine Learning and Its Applications in Orthopaedics: A Bibliometric Analysis.Cureus · 2025Review
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Obtaining frozen sections of bone tissue for intraoperative examination is challenging. To identify the bony edge of resection, orthopaedic oncologists therefore rely on pre-operative X-ray computed tomography or magnetic resonance imaging. However, these techniques do not allow for accurate diagnosis or for intraoperative confirmation of the tumour margins, and in bony sarcomas, they can lead to bone margins up to 10-fold wider (1,000-fold volumetrically) than necessary. Here, we show that real-time three-dimensional contour-scanning of tissue via ultraviolet photoacoustic microscopy in reflection mode can be used to intraoperatively evaluate undecalcified and decalcified thick bone specimens, without the need for tissue sectioning. We validate the technique with gold-standard haematoxylin-and-eosin histology images acquired via a traditional optical microscope, and also show that an unsupervised generative adversarial network can virtually stain the ultraviolet-photoacoustic-microscopy images, allowing pathologists to readily identify cancerous features. Label-free and slide-free histology via ultraviolet photoacoustic microscopy may allow for rapid diagnoses of bone-tissue pathologies and aid the intraoperative determination of tumour margins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.