ArticleNature communications2022
Structural characterization of a soil viral auxiliary metabolic gene product - a functional chitosanase.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Advances in phage-host interaction prediction: in silico method enhances the development of phage therapies.Briefings in bioinformatics · 2024Pooled it
- Microplastic and lead shift microbiomes enriching viral auxiliary metabolic genes for potential polylactic acid degradation.Communications biology · 2026Article
- Synergy and antagonism in a genome-scale model of metabolic hijacking by bacteriophages.Science advances · 2026Article
- Limited effect of short- to mid-term storage conditions on an Australian farmland soil RNA virome.Journal of virology · 2026Article
- HtPIP: High-throughput phage isolation platform increases diversity and reduces isolation time using multiple bacteria.Frontiers in microbiology · 2026Article
- Viral Dark Matter: Illuminating Protein Function, Ecology, and Biotechnological Promises.Biochemistry · 2025Review
- Effects of a novel Paraburkholderia phage IPK on the phenanthrene degradation efficiency of the PAH-degrading strain Paraburkholderia caledonica Bk.Biodegradation · 2025Article
- A call for caution in the biological interpretation of viral auxiliary metabolic genes.Nature microbiology · 2025Review
- Identification and profiling of novel metagenome assembled uncultivated virus genomes from human gut.Virology journal · 2025Article
- Viral Dark Matter: Illuminating Protein Function, Ecology, and Biotechnological Promises.ArXiv · 2025Article
- Genomic analysis of the novel Stenotrophomonas phage vB_Srh_LBjhp91a, isolated from a Karst cave in China.Archives of virology · 2025Article
- Tunturi virus isolates and metagenome-assembled viral genomes provide insights into the virome of Acidobacteriota in Arctic tundra soils.Microbiome · 2025Article
- SANS investigation of fungal loosenins reveals substrate-dependent impacts of protein action on the inter-microfibril arrangement of cellulosic substrates.Biotechnology for biofuels and bioproducts · 2025Article
- Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural Soils.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Viral communities in a pH>10 serpentinite-like environment: insight into diversity and potential roles in modulating the microbiomes by bioactive vitamin BApplied and environmental microbiology · 2024Article
- Genomic insights into redox-driven microbial processes for carbon decomposition in thawing Arctic soils and permafrost.mSphere · 2024Article
- A global atlas of soil viruses reveals unexplored biodiversity and potential biogeochemical impacts.Nature microbiology · 2024Article
- Exploring virus-host-environment interactions in a chemotrophic-based underground estuary.Environmental microbiome · 2024Article
- Zea mays genotype influences microbial and viral rhizobiome community structure.ISME communications · 2023Article
- Hi-C metagenome sequencing reveals soil phage-host interactions.Nature communications · 2023Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Metagenomics is unearthing the previously hidden world of soil viruses. Many soil viral sequences in metagenomes contain putative auxiliary metabolic genes (AMGs) that are not associated with viral replication. Here, we establish that AMGs on soil viruses actually produce functional, active proteins. We focus on AMGs that potentially encode chitosanase enzymes that metabolize chitin - a common carbon polymer. We express and functionally screen several chitosanase genes identified from environmental metagenomes. One expressed protein showing endo-chitosanase activity (V-Csn) is crystalized and structurally characterized at ultra-high resolution, thus representing the structure of a soil viral AMG product. This structure provides details about the active site, and together with structure models determined using AlphaFold, facilitates understanding of substrate specificity and enzyme mechanism. Our findings support the hypothesis that soil viruses contribute auxiliary functions to their hosts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.