Evidence map›Paper›PMID 36116790›Full record

ArticleDrug metabolism and disposition: the biological fate of chemicals2023

Gut Microbiome-Wide Search for Bacterial Azoreductases Reveals Potentially Uncharacterized Azoreductases Encoded in the Human Gut Microbiome.

Domenick J Braccia, Glory Minabou Ndjite, Ashley Weiss, Sophia Levy, Stephenie Abeysinghe, Xiaofang Jiang, Mihai Pop, Brantley Hall

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Drug metabolism and disposition: the biological fate of chemicals, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Domenick J BracciaCenter for Bioinformatics and Computational Biology (D.B., M.P., B.H.) and Departments of Cell Biology and Molecular Genetics (G.M.N., A.W., S.L., S.A., B.H.) and Computer Science (M.P.), University of Maryland, College Park, Maryland; and National Library of Medicine, National Institutes of Health, Bethesda, Maryland (X.J.).ORCID 0000-0002-4606-6254
Glory Minabou NdjiteCenter for Bioinformatics and Computational Biology (D.B., M.P., B.H.) and Departments of Cell Biology and Molecular Genetics (G.M.N., A.W., S.L., S.A., B.H.) and Computer Science (M.P.), University of Maryland, College Park, Maryland; and National Library of Medicine, National Institutes of Health, Bethesda, Maryland (X.J.).
Ashley WeissCenter for Bioinformatics and Computational Biology (D.B., M.P., B.H.) and Departments of Cell Biology and Molecular Genetics (G.M.N., A.W., S.L., S.A., B.H.) and Computer Science (M.P.), University of Maryland, College Park, Maryland; and National Library of Medicine, National Institutes of Health, Bethesda, Maryland (X.J.).
Sophia LevyCenter for Bioinformatics and Computational Biology (D.B., M.P., B.H.) and Departments of Cell Biology and Molecular Genetics (G.M.N., A.W., S.L., S.A., B.H.) and Computer Science (M.P.), University of Maryland, College Park, Maryland; and National Library of Medicine, National Institutes of Health, Bethesda, Maryland (X.J.).
Stephenie AbeysingheCenter for Bioinformatics and Computational Biology (D.B., M.P., B.H.) and Departments of Cell Biology and Molecular Genetics (G.M.N., A.W., S.L., S.A., B.H.) and Computer Science (M.P.), University of Maryland, College Park, Maryland; and National Library of Medicine, National Institutes of Health, Bethesda, Maryland (X.J.).
Xiaofang JiangCenter for Bioinformatics and Computational Biology (D.B., M.P., B.H.) and Departments of Cell Biology and Molecular Genetics (G.M.N., A.W., S.L., S.A., B.H.) and Computer Science (M.P.), University of Maryland, College Park, Maryland; and National Library of Medicine, National Institutes of Health, Bethesda, Maryland (X.J.).
Mihai PopCenter for Bioinformatics and Computational Biology (D.B., M.P., B.H.) and Departments of Cell Biology and Molecular Genetics (G.M.N., A.W., S.L., S.A., B.H.) and Computer Science (M.P.), University of Maryland, College Park, Maryland; and National Library of Medicine, National Institutes of Health, Bethesda, Maryland (X.J.).
Brantley HallCenter for Bioinformatics and Computational Biology (D.B., M.P., B.H.) and Departments of Cell Biology and Molecular Genetics (G.M.N., A.W., S.L., S.A., B.H.) and Computer Science (M.P.), University of Maryland, College Park, Maryland; and National Library of Medicine, National Institutes of Health, Bethesda, Maryland (X.J.) brantley@umd.edu.
National Institutes of Health · US

Funding

Algorithms and Software for the Assembly of Metagenomic DataR01AI100947 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI POP, MIHAI · 2013 to 2025
$7.4M
Comparative genomic analysis of bacteria speciesZIALM202010 · NLM · NATIONAL LIBRARY OF MEDICINE · PI JIANG, XIAOFANG · 2020 to 2025
$6.9M
NIAID NIH HHS R01 AI100947
6 · The paper itself

Abstract

The human gut is home to trillions of microorganisms that are responsible for the modification of many orally administered drugs, leading to a wide range of therapeutic outcomes. Prodrugs bearing an azo bond are designed to treat inflammatory bowel disease and colorectal cancer via microbial azo reduction, allowing for topical application of therapeutic moieties to the diseased tissue in the intestines. Despite the inextricable link between microbial azo reduction and the efficacy of azo prodrugs, the prevalence, abundance, and distribution of azoreductases have not been systematically examined across the gut microbiome. Here, we curated and clustered amino acid sequences of experimentally confirmed bacterial azoreductases and conducted a hidden Markov model-driven homolog search for these enzymes across 4644 genome sequences present in the representative Unified Human Gastrointestinal Genomes collection. We identified 1958 putative azo-reducing species, corroborating previous findings that azo reduction appears to be a ubiquitous function of the gut microbiome. However, through a systematic comparison of predicted and confirmed azo-reducing strains, we hypothesize the presence of uncharacterized azoreductases in 25 prominent strains of the human gut microbiome. Finally, we confirmed the azo reduction of Acid Orange 7 by multiple strains of

Indexed as

Gastrointestinal MicrobiomeInflammatory Bowel DiseasesProdrugsAzo CompoundsBacillotaBacteriaBenzenesulfonatesClostridiumHumansNADH, NADPH OxidoreductasesNitroreductases2-naphthol orangeAzo CompoundsazoreductaseBenzenesulfonatesNADH, NADPH OxidoreductasesNitroreductasesProdrugs

Identifiers

PMID36116790
PMCPMC11022935
OpenAlexW4296252121

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.