Evidence map›Paper›PMID 36114491›Full record

ArticleRespiratory research2022

Peroxisome proliferator-activated receptor gamma gene variants modify human airway and systemic responses to indoor dibutyl phthalate exposure.

Clarus Leung, Min Hyung Ryu, Anette Kocbach Bølling, Danay Maestre-Batlle, Christopher F Rider, Anke Hüls, Oscar Urtatiz, Julie L MacIsaac, Kevin Soon-Keen Lau, David Tse Shen Lin and 2 more

Registry-linked trialOpen access · goldAbstract readControlled Clinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Article in Respiratory research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02688478 (Effects of Phthalate Inhalation on Airway Immunology), which is not on this map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02688478 naunknown statusnot on this map

Effects of Phthalate Inhalation on Airway Immunology: A Controlled Human Exposure Study

TypeinterventionalSponsorUniversity of British ColumbiaRan2017 to 2021Enrolled20ConditionsAllergiesArmsPhthalate, Filtered Air, Allergen
3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Indoor air pollution and airway health.The Journal of allergy and clinical immunology · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Clarus LeungDepartment of Medicine, University of British Columbia, 7th Floor, 2775 Laurel St, VancouverVancouver, BC, V5Z1M9, Canada.
Min Hyung RyuDepartment of Medicine, University of British Columbia, 7th Floor, 2775 Laurel St, VancouverVancouver, BC, V5Z1M9, Canada.
Anette Kocbach BøllingDepartment of Air Pollution and Noise, Norwegian Institute of Public Health, Oslo, Norway.
Danay Maestre-BatlleDepartment of Medicine, University of British Columbia, 7th Floor, 2775 Laurel St, VancouverVancouver, BC, V5Z1M9, Canada.
Christopher F RiderDepartment of Medicine, University of British Columbia, 7th Floor, 2775 Laurel St, VancouverVancouver, BC, V5Z1M9, Canada.
Anke HülsDepartment of Epidemiology and Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA.
Oscar UrtatizDepartment of Medical Genetics, University of British Columbia-BC Children's Hospital Research Institute, Vancouver, BC, Canada.
Julie L MacIsaacDepartment of Medical Genetics, University of British Columbia-BC Children's Hospital Research Institute, Vancouver, BC, Canada.
Kevin Soon-Keen LauDepartment of Medicine, University of British Columbia, 7th Floor, 2775 Laurel St, VancouverVancouver, BC, V5Z1M9, Canada.
David Tse Shen LinDepartment of Medical Genetics, University of British Columbia-BC Children's Hospital Research Institute, Vancouver, BC, Canada.
Michael S KoborDepartment of Medical Genetics, University of British Columbia-BC Children's Hospital Research Institute, Vancouver, BC, Canada.
Chris CarlstenDepartment of Medicine, University of British Columbia, 7th Floor, 2775 Laurel St, VancouverVancouver, BC, V5Z1M9, Canada. carlsten@mail.ubc.ca.
University of British Columbia · CABC Children's Hospital · CAEmory University · USNorwegian Institute of Public Health · NO

Funding

Pilot Project ProgramP30ES019776 · NIEHS · EMORY UNIVERSITY · PI Carmen Joseph Marsit · 2013 to 2026
$22.6M
NIEHS NIH HHS P30 ES019776
6 · The paper itself

Abstract

backgroundSingle nucleotide polymorphisms (SNPs) of peroxisome proliferator-activated receptor gamma (PPAR-γ; gene: PPARG) and oxidative stress genes are associated with asthma risk. However, whether such variants modulate responses to dibutyl phthalate (DBP), a common plasticizer associated with increased asthma development, remains unknown. The purpose of this study is to investigate how SNPs in PPARG and oxidative stress genes, as represented by two separate genetic risk scores, modify the impact of DBP exposure on lung function and the airway and systemic response after an inhaled allergen challenge.

methodsWe conducted a double-blinded human crossover study with sixteen allergen-sensitized participants exposed for three hours to DBP and control air on distinct occasions separated by a 4-week washout. Each exposure was followed by an allergen inhalation challenge; subsequently, lung function was measured, and blood and bronchoalveolar lavage (BAL) were collected and analyzed for cell counts and allergen-specific immunoglobulin E (IgE). Genetic risk scores for PPAR-γ (P-GRS; weighted sum of PPARG SNPs rs10865710, rs709158, and rs3856806) and oxidative stress (OS-GRS; unweighted sum of 16 SNPs across multiple genes) were developed, and their ability to modify DBP effects were assessed using linear mixed-effects models.

resultsP-GRS and OS-GRS modified DBP effects on allergen-specific IgE in blood at 20 h (interaction effect [95% CI]: 1.43 [1.13 to 1.80], p = 0.005) and 3 h (0.99 [0.98 to 1], p = 0.03), respectively. P-GRS also modified DBP effects on Th2 cells in blood at 3 h (- 25.2 [- 47.7 to - 2.70], p = 0.03) and 20 h (- 39.1 [- 57.9 to - 20.3], p = 0.0005), and Th2 cells in BAL at 24 h (- 4.99 [- 8.97 to - 1.01], p = 0.02). An increasing P-GRS associated with reduced DBP effect on Th2 cells. Neither GRS significantly modified DBP effects on lung function parameters.

conclusionsPPAR-γ variants modulated several airway and systemic immune responses to the ubiquitous chemical plasticizer DBP. Our results suggest that PPAR-γ variants may play a greater role than those in oxidative stress-related genes in airway allergic responses to DBP.

trial registrationThis study reports results from The Phthalate-Allergen Immune Response Study that was registered on ClinicalTrials.gov with identification NCT02688478.

Indexed as

AsthmaDibutyl PhthalateAllergensCross-Over StudiesHumansImmunoglobulin EPlasticizersPPAR gammaAllergensDibutyl PhthalateImmunoglobulin EPlasticizersPPAR gammaAirway inflammationAllergen challengePeroxisome proliferator-activated receptor gammaPhthalatesPolymorphismPPAR-gamma

Identifiers

PMID36114491
PMCPMC9482266
OpenAlexW4296163095

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.