Evidence map›Paper›PMID 36114335›Full record

ArticleScientific reports2022

Discovery, isolation, heterologous expression and mode-of-action studies of the antibiotic polyketide tatiomicin from Amycolatopsis sp. DEM30355.

Bernhard Kepplinger, Lina Mardiana, Joseph Cowell, Stephanie Morton-Laing, Yousef Dashti, Corinne Wills, Emma C L Marrs, John D Perry, Joe Gray, Michael Goodfellow and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Synthetic and systems biotechnology · 2026
    Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Sensing of Antibiotic-Bacteria Interactions.Antibiotics (Basel, Switzerland) · 2023
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Bernhard KepplingerBiopharmaceutical Bioprocessing Technology Centre, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK. Bernhard.kepplinger@uwr.edu.pl.
Lina MardianaChemistry, School of Environmental and Natural Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Joseph CowellChemistry, School of Environmental and Natural Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Stephanie Morton-LaingChemistry, School of Environmental and Natural Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Yousef DashtiCentre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle Upon Tyne, NE2 4AX, UK.
Corinne WillsChemistry, School of Environmental and Natural Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Emma C L MarrsDepartment of Microbiology, Freeman Hospital, Newcastle upon Tyne, NE7 7DN, UK.
John D PerryDepartment of Microbiology, Freeman Hospital, Newcastle upon Tyne, NE7 7DN, UK.
Joe GrayPinnacle Laboratory, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle Upon Tyne, NE2 4AX, UK.
Michael GoodfellowBiology, School of Environmental and Natural Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Jeff ErringtonCentre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle Upon Tyne, NE2 4AX, UK.
Michael R ProbertChemistry, School of Environmental and Natural Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
William CleggChemistry, School of Environmental and Natural Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
Jonathan BogaertsMolecular Spectroscopy, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium.
Wouter HerreboutMolecular Spectroscopy, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium.
Nick E E AllenbyDemuris Limited, The Biosphere, Draymans Way, Newcastle Helix, Newcastle upon Tyne, NE4 5BX, UK. nick.allenby@demuris.co.uk.
Michael J HallChemistry, School of Environmental and Natural Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK. michael.hall@newcastle.ac.uk.

Funding

Wellcome Trust 209500Wellcome Trust 209500/Z/17/Z
6 · The paper itself

Abstract

A genomic and bioactivity informed analysis of the metabolome of the extremophile Amycolatopsis sp. DEM30355 has allowed for the discovery and isolation of the polyketide antibiotic tatiomicin. Identification of the biosynthetic gene cluster was confirmed by heterologous expression in Streptomyces coelicolor M1152. Structural elucidation, including absolute stereochemical assignment, was performed using complementary crystallographic, spectroscopic and computational methods. Tatiomicin shows antibiotic activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). Cytological profiling experiments suggest a putative antibiotic mode-of-action, involving membrane depolarisation and chromosomal decondensation of the target bacteria.

Indexed as

Methicillin-Resistant Staphylococcus aureusPolyketidesStreptomyces coelicolorAmycolatopsisAnti-Bacterial AgentsAnti-Bacterial AgentsPolyketides

Identifiers

PMID36114335
PMCPMC9481585

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.