Evidence map›Paper›PMID 36112238›Full record

ArticleMolecular and cellular biochemistry2023

Epigallocatechin-3-gallate inhibits the formation of neutrophil extracellular traps and suppresses the migration and invasion of colon cancer cells by regulating STAT3/CXCL8 pathway.

Zhuoxian Zhang, Qiuli Zhu, Siya Wang, Chao Shi

Abstract read
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In one paragraph

Article in Molecular and cellular biochemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 32 citations in OpenAlex.

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  17. Neutrophil extracellular traps in homeostasis and disease.Signal transduction and targeted therapy · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Zhuoxian ZhangDepartment of Oncology, Gaoxin Branch Of The First Affiliated Hospital Of Nanchang University, No.7889, Changdong avenue, Nanchang, 330006, Jiangxi Province, People's Republic of China.
Qiuli ZhuDepartment of Genetics, Gaoxin Branch Of The First Affiliated Hospital Of Nanchang University, Nanchang, 330006, Jiangxi Province, People's Republic of China.
Siya WangDepartment of Oncology, Gaoxin Branch Of The First Affiliated Hospital Of Nanchang University, No.7889, Changdong avenue, Nanchang, 330006, Jiangxi Province, People's Republic of China.
Chao ShiDepartment of Oncology, Gaoxin Branch Of The First Affiliated Hospital Of Nanchang University, No.7889, Changdong avenue, Nanchang, 330006, Jiangxi Province, People's Republic of China. shichao9066@163.com.ORCID http://orcid.org/0000-0002-2205-6457
Nanchang University · CN

Funding

Natural Science Foundation of Jiangxi Province 20192BAB205080
6 · The paper itself

Abstract

Colon cancer is a common malignant tumor of the digestive tract. Tea catechin exerts anti-tumor effects in colon cancer. This work aimed to determine the functions of epigallocatechin-3-gallate (EGCG), one of the main active components of Tea catechins, in the progression of colon cancer. In this work, enzyme-linked immune-sorbent assay, quantitative real-time PCR and western blotting was utilized to examine the levels of IL-1β, TNF-α, STAT3, p-STAT3 and CXCL8 in colon cancer patients and healthy controls. Compared with healthy controls, the levels of IL-1β and TNF-α were significantly increased in the peripheral blood of colon cancer patients, and the expression of STAT3, p-STAT3 and CXCL8 was elevated in the neutrophils derived from colon cancer patients. Moreover, neutrophils were treated with phorbol ester (PMA) or DNase I to induce or impede the formation of neutrophil extracellular traps (NETs). Both STAT3 overexpression and PMA treatment promoted the expression of CXCL8, myeloperoxidase (MPO) and citrullinated histone H3 (H3Cit) in the colon cancer-derived neutrophils, indicating that STAT3 overexpression facilitated the formation of NETs. STAT3 deficiency suppressed the formation of NETs, which consistent with the results of DNase I treatment. Transwell assay was utilized to detect the migration and invasion of colon cancer cell line SW480. EGCG treatment suppressed the formation of NETs and the expression of STAT3 and CXCL8 in the colon cancer-derived neutrophils, and then inhibited the migration and invasion of SW480 cells. In conclusion, this work demonstrated that EGCG inhibited the formation of NETs and subsequent suppressed the migration and invasion of colon cancer cells by regulating STAT3/CXCL8 signalling pathway. Thus, this study suggests that EGCG may become a potential drug for colon cancer therapy.

Indexed as

CatechinColonic NeoplasmsExtracellular TrapsDeoxyribonuclease IHumansNeutrophilsSTAT3 Transcription FactorTeaTumor Necrosis Factor-alphaCatechinDeoxyribonuclease Iepigallocatechin gallateSTAT3 protein, humanSTAT3 Transcription FactorTeaTumor Necrosis Factor-alphaColon cancerCXCL8Epigallocatechin-3-gallateInvasionMigrationNeutrophil extracellular trapsSTAT3

Identifiers

PMID36112238
OpenAlexW4296034322

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.