ArticlePhytochemical analysis : PCA2022
Analysis of 3-nitropropionic acid in Fabaceae plants by HPLC-MS/MS.
Article in Phytochemical analysis : PCA, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 10 citations in OpenAlex.
- Effective Extraction and Determination of 3-Nitropropanoic Acid in Sugarcane Samples Based on Magnetic Solid-Phase Extraction Coupled with HPLC-MS/MS.Foods (Basel, Switzerland) · 2026Article
- Barbigerone attenuates 3-nitropropionic acid-induced Huntington's disease-like neuropathology in rats via antioxidant, anti-inflammatory, and neuroprotective mechanisms.Scientific reports · 2025Article
- Analysis of 3-nitropropionic acid in Fabaceae plants by HPLC-MS/MS.Phytochemical analysis : PCA · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
introduction3-Nitropropionic acid (3-NPA) is a toxic compound that can accumulate in esterified form in the Fabaceae family. In the Lotae tribe, many species have been identified as 3-NPA producers (e.g., Securigera varia), while some of the genetically close Lotae plants were formerly reported as 3-NPA-free (e.g., Lotus corniculatus and Anthyllis vulneraria). These plants are used as forage and have a tradition in ethnomedicine, also, the extracts of A. vulneraria are used in cosmetics.
objectivesOur aim was to investigate the 3-NPA content of these selected Fabaceae species and to develop a validated quantitative method to evaluate 3-NPA concentrations in extracts of different herbal parts and cosmetic products. MATERIALS AND
methodsA UHPLC-ESI-Orbitrap-MS/MS method was applied for detection and identification of 3-NPA derivatives in the form of glucose esters. For the quantitative analysis, an optimized sample processing method was developed. The free 3-NPA content was determined using HPLC-ESI-MS/MS.
results3-NPA esters could be detected in all three species, but their quantity showed a high variation. S. varia contained 0.5-1.0 g/100 g of 3-NPA, while in L. corniculatus samples only trace quantities were detectable, below the LOQ (25 ng/ml). Most of the A. vulneraria samples showed similarly low concentrations, but one sample had 3-NPA levels comparable to S. varia. 3-NPA could not be detected in the tested cosmetics containing A. vulneraria extracts.
conclusionsUsing highly sensitive analytical methods, new 3-NPA-containing species were identified. The developed validated quantitative method is suitable for the determination of 3-NPA concentrations in herbal samples.
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