ArticleBiotechnology reports (Amsterdam, Netherlands)2022
Callus induction and regeneration in high-altitude Himalayan rice genotype SR4 via seed explant.
Article in Biotechnology reports (Amsterdam, Netherlands), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Overexpression of cryIAb gene for enhancing yellow stem borer (Scirpophaga incertulas) resistance using a marker-free double T-DNA system in Rojolele of Indonesia Rice cultivar.Journal, genetic engineering & biotechnology · 2026Article
- Multi-omics profiling reveals single-seed mutants of Ephedra saxatilis as dominant variants in high-altitude Xizang.BMC plant biology · 2025Article
- Protocol optimization for callus induction and shoot regeneration of Ethiopian rice varieties (Oryza sativa L.).BMC biotechnology · 2025Article
- In Vitro Propagation ofPlants (Basel, Switzerland) · 2025Article
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- Article
- A Versatile Protocol for Efficient Transformation and Regeneration in MegaMethods and protocols · 2023Article
- Plant regeneration from embryogenic callus-derived from immature leaves of Momordica charantia L.Heliyon · 2023Article
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Authors and funding
4 authors.
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Abstract
SR4 genotype of rice is high altitude Himalayan rice prone to various abiotic stresses such as cold stress and therefore gives a poor yield. An efficient protocol for callusing and regeneration via direct and indirect means was established using mature seeds as an explant which can be utilized for molecular studies for genetic advancement of Himalayan rice genotype SR4 through transformation. Highest frequency (96.6%) of callus induction was obtained on MS media 3.0 mg/L 2, 4-D. While maximum regeneration frequency (100%), number of shoots with maximum length 9.14 ± 0.204 (cm) from callus was recovered from MS media amended with 5.0 mg/L BAP in combination with 0.5 mg/L NAA with highest number of shoots having an average shoot length 9.14 ± 0.204 (cm) after four weeks of culture. Direct multiple shoot regeneration from seed explants was obtained using various concentrations of TDZ and BAP with highest regeneration frequency was observed on MS media fortified with 6 mg/L of TDZ with maximum number of shoots. The shoots developed roots on MS media supplemented with 0.6 mg/L IBA.
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