Evidence map›Paper›PMID 36109385›Full record

ArticleApplied microbiology and biotechnology2022

Protein engineering for feedback resistance in 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.

Kumaresan Jayaraman, Natalia Trachtmann, Georg A Sprenger, Holger Gohlke

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 23 citations in OpenAlex.

  1. Strategy for enhanced production of A40926B0 inSynthetic and systems biotechnology · 2027
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Kumaresan Jayaraman *Institut für Pharmazeutische und Medizinische Chemie, Heinrich-Heine-Universität Düsseldorf, 40225, Düsseldorf, Germany.ORCID http://orcid.org/0000-0002-4891-5571
Natalia Trachtmann *Institute of Microbiology, University of Stuttgart, Allmandring 31, 70569, Stuttgart, Germany.
Georg A SprengerInstitute of Microbiology, University of Stuttgart, Allmandring 31, 70569, Stuttgart, Germany. georg.sprenger@imb.uni-stuttgart.de.ORCID http://orcid.org/0000-0002-7879-8978
Holger GohlkeInstitut für Pharmazeutische und Medizinische Chemie, Heinrich-Heine-Universität Düsseldorf, 40225, Düsseldorf, Germany. gohlke@uni-duesseldorf.de.ORCID http://orcid.org/0000-0001-8613-1447
University of Stuttgart · DEForschungszentrum Jülich · DEHeinrich Heine University Düsseldorf · DE

Funding

Bundesministerium für Ernährung und Landwirtschaft (BMEL) 22010215 ("Bio4PURPro")
6 · The paper itself

Abstract

The shikimate pathway delivers aromatic amino acids (AAAs) in prokaryotes, fungi, and plants and is highly utilized in the industrial synthesis of bioactive compounds. Carbon flow into this pathway is controlled by the initial enzyme 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHPS). AAAs produced further downstream, phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp), regulate DAHPS by feedback inhibition. Corynebacterium glutamicum, the industrial workhorse for amino acid production, has two isoenzymes of DAHPS, AroF (Tyr sensitive) and AroG (Phe and Tyr sensitive). Here, we introduce feedback resistance against Tyr in the class I DAHPS AroF (AroF

Indexed as

3-Deoxy-7-Phosphoheptulonate SynthaseEscherichia coliAmino Acids, AromaticCarbonFeedbackIsoenzymesPhenylalaninePhosphatesProtein EngineeringTryptophanTyrosine3-Deoxy-7-Phosphoheptulonate SynthaseAmino Acids, AromaticCarbonIsoenzymesPhenylalaninePhosphatesTryptophanTyrosineDAHP synthaseEnzyme engineeringFeedback resistanceProtein stabilityShikimate pathway

Identifiers

PMID36109385
PMCPMC9529685
OpenAlexW4296026867

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.