ArticleeLife2022
Murine blastocysts generated by in vitro fertilization show increased Warburg metabolism and altered lactate production.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
24 citing papers in PubMed, 26 citations in OpenAlex.
- Ultra-low oxygen tension duringbioRxiv : the preprint server for biology · 2026Article
- Extracellular Vesicles From Human Fallopian Tubes Enhance IVF Embryo Development and Contain Functional Proteins Including YWHAZ.Journal of extracellular vesicles · 2026Article
- Label-free candidate dOCM biomarkers associated with blastocyst quality using total cell count as a proxy.Biomedical optics express · 2026Article
- When less really is more: reducing embryo manipulation to lower metabolic stress and improving long term outcomes.Reproduction, fertility, and development · 2026Review
- Metabolic Plasticity in Embryogenesis Throughout the Lens of NADMethods in molecular biology (Clifton, N.J.) · 2026Review
- Reproductive fluids, commercial media, and organoids: bridging the gap in IVF culture systems.Human reproduction open · 2026Review
- Crosstalk between H3K4me3 and oxidative stress is a potential target for the improvement of ART-derived embryos.Nature communications · 2025Article
- Proteomic and metabolomic reveals abnormalities in metabolic processes, epigenetic modifications, oxidative stress, embryonic aneuploidy and implantation in mouse blastocysts derived from in vitro fertilization.Journal of ovarian research · 2025Article
- Impact of embryo culture and day of embryo transfer on the cardiometabolic health of adult mice†.Biology of reproduction · 2025Article
- Overload of Glucose Metabolism as Initiating Factor in Diabetic Embryopathy and Prevention by Glyoxalase 1 Inducer Dietary Supplement.Antioxidants (Basel, Switzerland) · 2025Article
- Metabolic regulation of key developmental events during mammalian embryogenesis.Nature cell biology · 2025Review
- Dynamic Proteome Landscape During Preimplantation Human Embryo Development and Trophectoderm Stem Cell-Differentiation.Proteomics · 2025Article
- Association Between Human Embryo Culture Conditions, Cryopreservation, and the Potential Risk of Birth Defects in Children Conceived Through Assisted Reproduction Technology.Medicina (Kaunas, Lithuania) · 2025Review
- Calcium signals shape metabolic control of H3K27ac and H3K18la to regulate EGA.bioRxiv : the preprint server for biology · 2025Article
- Effects of Follicular Fluid and Serum Supplementation on Cumulus Cell Expansion and Nuclear Progression of Guinea Pig Oocytes, Using a Baseline Medium Established with Bovine Oocytes.Animals : an open access journal from MDPI · 2025Article
- Chloroquine mitigates long-term effects of in vitro culture in mouse embryos.Frontiers in cell and developmental biology · 2025Article
- Proteomic and metabolomic insights into oxidative stress response activation in mouse embryos generated byHuman reproduction open · 2025Article
- Linking the Warburg effect to endometrial receptivity: metabolic parallels in embryo implantation.Frontiers in cell and developmental biology · 2025Review
- The composition of commercially available human embryo culture media.Human reproduction (Oxford, England) · 2025Article
- Understanding the intersection between placental development and cancer: Lessons from the tumor suppressor BAP1.Communications biology · 2024Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In vitro fertilization (IVF) has resulted in the birth of over 8 million children. Although most IVF-conceived children are healthy, several studies suggest an increased risk of altered growth rate, cardiovascular dysfunction, and glucose intolerance in this population compared to naturally conceived children. However, a clear understanding of how embryonic metabolism is affected by culture condition and how embryos reprogram their metabolism is unknown. Here, we studied oxidative stress and metabolic alteration in blastocysts conceived by natural mating or by IVF and cultured in physiologic (5%) or atmospheric (20%) oxygen. We found that IVF-generated blastocysts manifest increased reactive oxygen species, oxidative damage to DNA/lipid/proteins, and reduction in glutathione. Metabolic analysis revealed IVF-generated blastocysts display decreased mitochondria respiration and increased glycolytic activity suggestive of enhanced Warburg metabolism. These findings were corroborated by altered intracellular and extracellular pH and increased intracellular lactate levels in IVF-generated embryos. Comprehensive proteomic analysis and targeted immunofluorescence showed reduction of lactate dehydrogenase-B and monocarboxylate transporter 1, enzymes involved in lactate metabolism. Importantly, these enzymes remained downregulated in the tissues of adult IVF-conceived mice, suggesting that metabolic alterations in IVF-generated embryos may result in alteration in lactate metabolism. These findings suggest that alterations in lactate metabolism are a likely mechanism involved in genomic reprogramming and could be involved in the developmental origin of health and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.