ArticleBiology of reproduction2022
Successful in vitro fertilization in the horse: production of blastocysts and birth of foals after prolonged sperm incubation for capacitation†.
Article in Biology of reproduction, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 62 citations in OpenAlex.
- One Embryology: impactful connections between human- and wildlife-assisted reproduction.Journal of assisted reproduction and genetics · 2026Review
- Sex determination and genetic screening of equine embryos with whole genome amplification and real-time PCR.The Journal of reproduction and development · 2026Article
- Comparative Evaluation of Follicular Flushing Frequency and Scraping Time During Ovum Pick-Up in Mares: Effects on Oocyte Recovery Rate and Technical Considerations.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Sperm energy-restriction and recovery enhances equine sperm function and embryo development after ICSI.Journal of animal science · 2026Article
- Catalase in Unexpected Places: Revisiting HAntioxidants (Basel, Switzerland) · 2025Article
- Equine in vitro fertilization with frozen-thawed semen is associated with shortened pre-incubation time and modified capacitation-related changes.Biology of reproduction · 2025Article
- Current and Emerging Advanced Techniques for Breeding Donkeys and Mules.Animals : an open access journal from MDPI · 2025Review
- The Influence of Aspiration Pressure, Follicle Flushing Method and Needle Rotation During Single-Operator OPU Technique on Oocyte Recovery and Embryo Production in the Mare.Animals : an open access journal from MDPI · 2025Article
- Maturational competence of equine oocytes is associated with alterations in their 'cumulome'.Molecular human reproduction · 2024Article
- Emerging Contributions of Pluripotent Stem Cells to Reproductive Technologies in Veterinary Medicine.Journal of developmental biology · 2024Review
- Genome-wide equine preimplantation genetic testing enabled by simultaneous haplotyping and copy number detection.Scientific reports · 2024Article
- Mapping the lipidomic secretome of the early equine embryo.Frontiers in veterinary science · 2024Article
- Comparative reproduction of the female horse, elephant and rhinoceros: implications for advancing Assisted Reproductive Technologies (ART).Reproduction & fertility · 2023Article
- Progesterone and Androstenedione Are Important Follicular Fluid Factors Regulating Porcine Oocyte Maturation Quality.Animals : an open access journal from MDPI · 2023Article
- Postmortem Collection of Gametes for the Conservation of Endangered Mammals: A Review of the Current State-of-the-Art.Animals : an open access journal from MDPI · 2023Review
- The sperm mitochondria: clues and challenges.Animal reproduction · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Methods for standard in vitro fertilization have been difficult to establish in the horse. We evaluated whether prolonged sperm pre-incubation would support subsequent fertilization. Fresh sperm were pre-incubated with penicillamine, hypotaurine, and epinephrine (PHE) for 22 h. Co-incubation of cumulus-oocyte complexes (COCs) for 6 h yielded 43% fertilization; culture of presumptive embryos yielded 21% blastocysts. Sperm incubated similarly, but without PHE, did not fertilize oocytes. Use of extended semen in the system yielded 54% blastocysts and was applied in subsequent experiments. Transfer of three in vitro fertilization-produced blastocysts to recipient mares resulted in birth of three normal foals. When sperm were pre-incubated for 22 h, 47-79% of oocytes were fertilized after 1 h of co-incubation. Sperm pre-incubated for 15 min or 6 h before co-incubation yielded no fertilization at 1 h, suggesting that capacitation in this system requires between 6 and 22 h. Sperm assessed after 15 min, 6 h, or 22 h pre-incubation showed increasing protein tyrosine phosphorylation of the midpiece, equatorial band, and apical head; this pattern differed from that induced by high pH conditions and may denote functional equine sperm capacitation. Use of the final devised system, i.e., extended semen, with 22 h of sperm pre-incubation and 3 h of COC co-incubation, yielded 90% fertilization with a blastocyst rate of 74%. This is the first report of efficient and repeatable standard in vitro fertilization in the horse and the first report of in vitro production of blastocysts and resulting foals after in vitro fertilization.
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