Evidence map›Paper›PMID 36104808›Full record

ArticleBiotechnology for biofuels and bioproducts2022

Both levoglucosan kinase activity and transport capacity limit the utilization of levoglucosan in Saccharomyces cerevisiae.

Mengdan Yang, Tiandi Wei, Kai Wang, Liqun Jiang, Dihao Zeng, Xinhua Sun, Weifeng Liu, Yu Shen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology for biofuels and bioproducts, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 64% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Mengdan YangState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China.
Tiandi WeiState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China.
Kai WangState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China.
Liqun JiangGuangdong Engineering Laboratory of Biomass High-Value Utilization, Guangzhou Key Laboratory of Biomass Comprehensive Utilization, Guangdong Plant Fiber Comprehensive Utilization Engineering Technology Research and Development Center, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou, 510640, China.
Dihao ZengState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China.
Xinhua SunState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China.
Weifeng LiuState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China.
Yu ShenState Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, China. shenyu@sdu.edu.cn.
Shandong University · CNGuangdong Academy of Sciences · CN

Funding

National Key Research and Development Program of China 2021YFC2101303National Natural Science Foundation of China 32170039State Key Laboratory of Microbial Technology Open Projects Fund M2019-10
6 · The paper itself

Abstract

Manufacturing fuels and chemicals from cellulose materials is a promising strategy to achieve carbon neutralization goals. In addition to the commonly used enzymatic hydrolysis by cellulase, rapid pyrolysis is another way to degrade cellulose. The sugar obtained by fast pyrolysis is not glucose, but rather its isomer, levoglucosan (LG). Here, we revealed that both levoglucosan kinase activity and the transportation of levoglucosan are bottlenecks for LG utilization in Saccharomyces cerevisiae, a widely used cell factory. We revealed that among six heterologous proteins that had levoglucosan kinase activity, the 1,6-anhydro-N-acetylmuramic acid kinase from Rhodotorula toruloides was the best choice to construct levoglucosan-utilizing S. cerevisiae strain. Furthermore, we revealed that the amino acid residue Q341 and W455, which were located in the middle of the transport channel closer to the exit, are the sterically hindered barrier to levoglucosan transportation in Gal2p, a hexose transporter. The engineered yeast strain expressing the genes encoding the 1,6-anhydro-N-acetylmuramic acid kinase from R. toruloides and transporter mutant Gal2p

Indexed as

1,6-Anhydro-N-acetylmuramic acid kinaseGal2pLevoglucosanSaccharomyces cerevisiaeTransporter

Identifiers

PMID36104808
PMCPMC9476349
OpenAlexW4295700778

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.