ArticleNature communications2022
Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 59 citations in OpenAlex.
- Lights up on the embryonic dance: tools and applications of optogenetics in developmental biology.Genes & development · 2026Review
- Optical Control of Actin Network Assembly on the Supported Lipid Bilayer.Bio-protocol · 2026Article
- Engineering development: From the repressilator and toggle switch to synthetic developmental biology.Developmental biology · 2025Review
- Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins.Nature communications · 2025Article
- Engineering filamentous myosins for optical control of contractility.bioRxiv : the preprint server for biology · 2025Article
- Designer mammalian living materials through genetic engineering.Bioactive materials · 2025Review
- Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion.Developmental biology · 2025Review
- Bioengineering innovations for neural organoids with enhanced fidelity and function.Cell stem cell · 2025Review
- Measuring and manipulating mechanical forces during development.Nature cell biology · 2025Review
- The dynamics of tubulogenesis in development and disease.Development (Cambridge, England) · 2025Review
- Article
- Image-guided optogenetic spatiotemporal tissue patterning using μPatternScope.Nature communications · 2024Article
- Genetically-stable engineered optogenetic gene switches modulate spatial cell morphogenesis in two- and three-dimensional tissue cultures.Nature communications · 2024Article
- Borg5 restricts contractility and motility in epithelial MDCK cells.Journal of cell science · 2024Article
- The Role of Biophysical Factors in Organ Development: Insights from Current Organoid Models.Bioengineering (Basel, Switzerland) · 2024Review
- Advanced 3D imaging and organoid bioprinting for biomedical research and therapeutic applications.Advanced drug delivery reviews · 2024Review
- Photoactivation of LOV domains with chemiluminescence.Chemical science · 2024Article
- Mapping morphogenesis and mechanics in embryo models.Nature methods · 2023Article
- Light-Induced Nanoscale Deformation in Azobenzene Thin Film Triggers Rapid Intracellular CaAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- 'Chip'-ing away at morphogenesis - application of organ-on-chip technologies to study tissue morphogenesis.Journal of cell science · 2023Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emerging field of synthetic developmental biology proposes bottom-up approaches to examine the contribution of each cellular process to complex morphogenesis. However, the shortage of tools to manipulate three-dimensional (3D) shapes of mammalian tissues hinders the progress of the field. Here we report the development of OptoShroom3, an optogenetic tool that achieves fast spatiotemporal control of apical constriction in mammalian epithelia. Activation of OptoShroom3 through illumination in an epithelial Madin-Darby Canine Kidney (MDCK) cell sheet reduces the apical surface of the stimulated cells and causes displacements in the adjacent regions. Light-induced apical constriction provokes the folding of epithelial cell colonies on soft gels. Its application to murine and human neural organoids leads to thickening of neuroepithelia, apical lumen reduction in optic vesicles, and flattening in neuroectodermal tissues. These results show that spatiotemporal control of apical constriction can trigger several types of 3D deformation depending on the initial tissue context.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.