ArticleBiology2022
Revealing the Microbiome of Four Different Thermal Springs in Turkey with Environmental DNA Metabarcoding.
Article in Biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Microbiome characterization of alpine water springs for human consumption reveals site- and usage-specific microbial signatures.Frontiers in microbiology · 2022Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
One of the most significant challenges for detecting microbial life in thermal springs by conventional techniques such as culturing is these places' physicochemical (temperature, heavy metal content, pH, etc.) conditions. Data from several studies suggest that high-throughput DNA sequencing technologies can be used to perform more accurate and detailed microbiome analyses. The primary aim of this paper was to determine the microbiome in the thermal source by metabarcoding environmental DNA isolated from four different sources and reveal the reflection of differences caused by temperature and chemical content on the microbiome. DNA was extracted from water filtered with enclosed filters and using the Illumina high-throughput sequencing platform, V3 and V4 regions of the 16S rRNA gene were sequenced. The results showed a correlation between physicochemical conditions and microorganism composition of four different thermal springs. Springs with extremely high temperature (89-90 °C) were dominated by hyperthermophiles such as
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