Evidence map›Paper›PMID 36101358›Full record

ArticleBiology2022

Comparison of Traditional and Next-Generation Approaches for Uncovering Phytoplasma Diversity, with Discovery of New Groups, Subgroups and Potential Vectors.

Valeria Trivellone, Yanghui Cao, Christopher H Dietrich

Open access · goldAbstract read
In one paragraph

Article in Biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
4.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 24 citations in OpenAlex.

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  9. First record of the non-nativeBiodiversity data journal · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Valeria TrivelloneIllinois Natural History Survey, Prairie Research Institute, University of Illinois at Urbana Champaign, Champaign, IL 61820, USA.ORCID 0000-0003-1415-4097
Yanghui CaoIllinois Natural History Survey, Prairie Research Institute, University of Illinois at Urbana Champaign, Champaign, IL 61820, USA.
Christopher H DietrichIllinois Natural History Survey, Prairie Research Institute, University of Illinois at Urbana Champaign, Champaign, IL 61820, USA.ORCID 0000-0003-4005-4305
University of Illinois Urbana-Champaign · US

Funding

U.S. National Science Foundation DEB 16-39601
6 · The paper itself

Abstract

Despite several decades' effort to detect and identify phytoplasmas (Mollicutes) using PCR and Sanger sequencing focusing on diseased plants, knowledge of phytoplasma biodiversity and vector associations remains highly incomplete. To improve protocols for documenting phytoplasma diversity and ecology, we used DNA extracted from phloem-feeding insects and compared traditional Sanger sequencing with a next-generation sequencing method, Anchored Hybrid Enrichment (AHE) for detecting and characterizing phytoplasmas. Among 22 of 180 leafhopper samples that initially tested positive for phytoplasmas using qPCR, AHE yielded phytoplasma

Indexed as

anchored hybrid enrichmentbiodiversitybiorepositorynested PCRsanger sequencing

Identifiers

PMID36101358
PMCPMC9312118
OpenAlexW4283688742

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.