Evidence map›Paper›PMID 36100522›Full record

ArticleTrends in biochemical sciences2023

Snapshots of a tiny ancestral nuclease of Cas9.

Zhonggang Hou, Renke Tan, Yan Zhang

Abstract readComment
PubMed Publisher
In one paragraph

Article in Trends in biochemical sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Intervertebral disc degeneration and inflammatory microenvironment: expression, pathology, and therapeutic strategies.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2023
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Zhonggang HouDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Renke TanDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
Yan ZhangDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: yzhangbc@med.umich.edu.

Funding

Generation of DNA memory by bacterial CRISPR-Cas9 systemsR35GM137883 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Yan Zhang · 2020 to 2026
$3.0M
NIGMS NIH HHS R35 GM137883
6 · The paper itself

Abstract

High-resolution structures solved by Schuler et al. shed light on how Cas9's evolutionary ancestor IscB operates as an RNA-guided nuclease. With only two-fifths the size of Cas9, IscB holds great promise for alleviating the cargo size constraint of in vivo CRISPR delivery.

Indexed as

CRISPR-Cas SystemsRNAEndonucleasesEndonucleasesRNACas9CRISPRgenome editingIscBRNA-guided nuclease

Identifiers

PMID36100522

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.