ArticleJournal of advanced research2022
Consensus co-expression network analysis identifies AdZAT5 regulating pectin degradation in ripening kiwifruit.
Article in Journal of advanced research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 39 citations in OpenAlex.
- CaZAT5 delays the flowering time in tomato and affects pollen viability and anther dehiscence.PLoS genetics · 2026Article
- Conservation and divergence of abiotic stress-responsive gene co-expression networks in diploid and tetraploid cotton.Frontiers in plant science · 2026Article
- Article
- Transcriptome Analysis Unveils the Molecular Mechanisms of Ethylene-Induced Ready-to-Eat Kiwifruit-Picking Ripening.Foods (Basel, Switzerland) · 2025Article
- The transcription factors AdNAC3 and AdMYB19 regulate kiwifruit ripening through brassinosteroid and ethylene signaling networks.Plant physiology · 2025Article
- GWAS and WGCNA analysis uncover candidate genes associated with drought inFrontiers in plant science · 2025Article
- Haplotype-resolved genome assembly provides insights into evolutionary history of the Actinidia arguta tetraploid.Molecular horticulture · 2024Article
- The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit.Plant physiology · 2023Article
- How sweet! Transcription factor CitZAT5 regulates CitSUS5 and CitSWEET6 to promote sugar accumulation in citrus fruit.Plant physiology · 2023Article
- Identification and Characterization of Long Non-Coding RNAs: Implicating Insights into Their Regulatory Role in Kiwifruit Ripening and Softening during Low-Temperature Storage.Plants (Basel, Switzerland) · 2023Article
- Role of integrated omics in unravelling fruit stress and defence responses during postharvest: A review.Food chemistry. Molecular sciences · 2022Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionCell wall degradation and remodeling is the key factor causing fruit softening during ripening.
objectivesTo explore the mechanism underlying postharvest cell wall metabolism, a transcriptome analysis method for more precious prediction on functional genes was needed.
methodsKiwifruits treated by ethylene (a conventional and effective phytohormone to accelerate climacteric fruit ripening and softening as kiwifruits) or air were taken as materials. Here, Consensus Coexpression Network Analysis (CCNA), a procedure evolved from Weighted Gene Co-expression Network Analysis (WGCNA) package in R, was applied and generated 85 consensus clusters from twelve transcriptome libraries. Advanced and comprehensive modifications were achieved by combination of CCNA and WGCNA with introduction of physiological traits, including firmness, cell wall materials, cellulose, hemicellulose, water soluble pectin, covalent binding pectin and ionic soluble pectin.
resultsAs a result, six cell wall metabolisms related structural genes AdGAL1, AdMAN1, AdPL1, AdPL5, Adβ-Gal5, AdPME1 and four transcription factors AdZAT5, AdDOF3, AdNAC083, AdMYBR4 were identified as hub candidate genes for pectin degradation. Dual-luciferase system and electrophoretic mobility shift assays validated that promoters of AdPL5 and Adβ-Gal5 were recognized and trans-activated by transcription factor AdZAT5. The relatively higher enzyme activities of PL and β-Gal were observed in ethylene treated kiwifruit, further emphasized the critical roles of these two pectin related genes for fruit softening. Moreover, stable transient overexpression AdZAT5 in kiwifruit significantly enhanced AdPL5 and Adβ-Gal5 expression, which confirmed the in vivo regulations between transcription factor and pectin related genes.
conclusionThus, modification and application of CCNA would be powerful for the precious phishing the unknown regulators. It revealed that AdZAT5 is a key factor for pectin degradation by binding and regulating effector genes AdPL5 and Adβ-Gal5.
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