Evidence map›Paper›PMID 36098933›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2022

Streptococcus mutans glutamate binding protein (GlnH) as antigen target for a mucosal anti-caries vaccine.

Gisela de Souza Pereira, Milene Tavares Batista, Nayara Fernanda Barros Dos Santos, Hélic Moreira Passos, Dalva Adelina da Silva, Ewerton Lucena Ferreira, Luís Carlos de Souza Ferreira, Rita de Cássia Café Ferreira

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PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 7 citations in OpenAlex.

  1. Pooled it
  2. Design of a Novel Peptide-Based Vaccine TargetingInternational journal of dentistry · 2026
    Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Gisela de Souza PereiraDepartment of Microbiology, Biomedical Science Institute, University of São Paulo, São Paulo, SP, 137405508-900s, Brazil.
Milene Tavares BatistaDepartment of Microbiology, Biomedical Science Institute, University of São Paulo, São Paulo, SP, 137405508-900s, Brazil.ORCID http://orcid.org/0000-0002-2102-244X
Nayara Fernanda Barros Dos SantosDepartment of Microbiology, Biomedical Science Institute, University of São Paulo, São Paulo, SP, 137405508-900s, Brazil.
Hélic Moreira PassosDepartment of Microbiology, Biomedical Science Institute, University of São Paulo, São Paulo, SP, 137405508-900s, Brazil.
Dalva Adelina da SilvaDepartment of Microbiology, Biomedical Science Institute, University of São Paulo, São Paulo, SP, 137405508-900s, Brazil.
Ewerton Lucena FerreiraDepartment of Microbiology, Biomedical Science Institute, University of São Paulo, São Paulo, SP, 137405508-900s, Brazil.
Luís Carlos de Souza FerreiraDepartment of Microbiology, Biomedical Science Institute, University of São Paulo, São Paulo, SP, 137405508-900s, Brazil.
Rita de Cássia Café FerreiraDepartment of Microbiology, Biomedical Science Institute, University of São Paulo, São Paulo, SP, 137405508-900s, Brazil. ritacafe@usp.br.
Universidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn recent years, several studies have demonstrated that bacterial ABC transporters present relevant antigen targets for the development of vaccines against bacteria such as Streptococcus pneumoniae and Enterococcus faecalis. In Streptococcus mutans, the glutamate transporter operon (glnH), encoding an ABC transporter, is associated with acid tolerance and represents an important virulence-associated factor for the development of dental caries.

resultsIn this study, we generated a recombinant form of the S. mutans GlnH protein (rGlnH) in Bacillus subtilis. Mice immunized with this protein antigen elicited strong antigen-specific antibody responses after sublingual administration of a vaccine formulation containing a mucosal adjuvant, a non-toxic derivative of the heat-labile toxin (LTK63) originally produced by enterotoxigenic Escherichia coli (ETEC) strains. Serum anti-rGlnH antibodies reduced adhesion of S. mutans to the oral cavity of naïve mice. Moreover, mice actively immunized with rGlnH were partially protected from oral colonization after exposure to the S. mutans NG8 strain.

conclusionsOur results indicate that S. mutans rGlnH is a potential target antigen capable of inducing specific and protective antibody responses after immunization. Overall, these observations raise the prospect of the development of mucosal anti-caries vaccines.

Indexed as

Dental CariesStreptococcus mutansAnimalsAntibodies, BacterialCariostatic AgentsCarrier ProteinsGlutamic AcidMiceProteinsSalivaAntibodies, BacterialCariostatic AgentsCarrier ProteinsGlutamic AcidProteinsABC transportersAnti-caries vaccinesDental cariesGlnHLTK63Mucosal adjuvantStreptococcus mutans

Identifiers

PMID36098933
PMCPMC9679091
OpenAlexW4295455526

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.