ArticleInternational journal of implant dentistry2022
Exploration of proper heating protocol for injectable horizontal platelet-rich fibrin gel.
Article in International journal of implant dentistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Effects of albumin-platelet-rich fibrin as an adjunct to non-surgical periodontal therapy in patients with periodontitis: a randomized controlled clinical trial.BMC oral health · 2026Trial
- Comparing two types of platelet-rich fibrin membranes in the management of dehiscence defects with simultaneous implant placement in the posterior mandible: a randomized controlled trial.BMC oral health · 2026Trial
- Trial
- Efficacy of injectable platelet-rich fibrin (i-PRF) for gingival phenotype modification: a split-mouth randomized controlled clinical trial.Clinical oral investigations · 2023Trial
- Clinical effectiveness of albumin-enriched platelet-rich fibrin (Alb-PRF) in periodontal and peri-implant regenerative surgery: a systematic review.Odontology · 2026Review
- Impact of Thermal Treatment on Growth Factor Levels in Injectable Horizontal Platelet-Rich Fibrin: An In Vitro Study.Clinical and experimental dental research · 2026Article
- Antibacterial Activity of Fresh and Autoclaved Platelet-Rich Fibrin on Pseudomonas aeruginosa: An In Vitro Study.Journal of clinical laboratory analysis · 2026Article
- Biological impact of adding albumin and silver nanoparticles to concentrated growth factors.BMC oral health · 2026Article
- Advantages of horizontal centrifugation of platelet-rich fibrin in regenerative medicine and dentistry.Periodontology 2000 · 2025Review
- Tensile Strength, Cellular Content and Degradation properties in three generations of concentrated growth factors.BMC oral health · 2025Article
- Article
- Comparison of the effect of albumin with platelet-rich fibrin (Alb-PRF) gel and hyaluronic acid gel injection on interdental papilla reconstruction: A randomized clinical trial.Journal of advanced periodontology & implant dentistry · 2024Article
- Technical considerations in obtaining platelet rich fibrin for clinical and periodontal research.Journal of oral biology and craniofacial researchArticle
- RE: Technical considerations in obtaining platelet-rich fibrin for clinical and periodontal research.Journal of oral biology and craniofacial researchArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposePlatelet-rich fibrin (PRF) has been proposed as promising biomaterials with the advantages of host accumulation of platelets and leukocytes with entrapment of growth factors and fibrin scaffold. However, limitations including fast resorption rate (~ 2 weeks) restricts its clinical application. Recent studies have demonstrated heating treatment can prolong PRF degradation. Current published articles used the method of 75 °C for 10 min to obtain longer degradation, while few studies investigated the most suitable temperature for heating horizontal PRF. Our present study was to discover and confirm the optimum temperature for heat treatment before obtaining H-PRF gels by investigating their structure, mechanical properties, and bioactivity of the H-PRF gels after heating treatment.
methodsIn the present study, 2-mL upper layer of horizontal PRF was collected and heated at 45 °C, 60 °C, 75 °C, and 90 °C to heat 2-mL upper layer of horizontal PRF for 10 min before mixing with the 2-mL lower layer horizontal PRF. The weight, solidification time and the degradation properties were subsequently recorded. Scanning electron microscopy (SEM) and rheologic tests were carried out to investigate the microstructure and rheologic properties of each H-PRF gel. The biological activity of each H-PRF gel was also evaluated using live/dead staining.
resultsH-PRF gel prepared at 75 °C for 10 min had the fast solidification period (over a tenfold increase than control) as well as the best resistance to degradation. The number of living cells in H-PRF gel is greater than 90%. SEM showed that H-PRF gel becomes denser as the heating temperature increases, and rheologic tests also revealed that the heat treatment improved the mechanical properties of H-PRF gels when compared to non-heated control group. Future clinical studies are needed to further support the clinical application of H-PRF gels in tissue regeneration procedures.
conclusionsOur results demonstrated that the H-PRF gel obtained at 75 °C for 10 min could produce a uniform, moldable gel with a short time for solidification time, great rheologic behavior and, high percent of live cells in PRF gel. A promising use of the commonly utilized PRF gel was achieved facilitating tissue regeneration and preventing degradation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.