Evidence map›Paper›PMID 36098848›Full record

ArticleArchives of microbiology2022

Probiotic potential and immunomodulatory properties in Enterococcus faecium GMB24 and Enterococcus hirae SMB16 isolated from goat and sheep milk.

Kamni Rajput, Ramesh Chandra Dubey, Ashwani Kumar

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. EvaluatingFood science & nutrition · 2024
    Article
  8. Article
  9. In Vitro Interaction betweenMicroorganisms · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Symbiotic Supplementation (Cell transplantation
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kamni RajputDepartment of Botany and Microbiology, Gurukula Kangri (Deemed to Be University), Haridwar, 249404, India. kamnirajput20@gmail.com.ORCID http://orcid.org/0000-0003-1511-3035
Ramesh Chandra DubeyDepartment of Botany and Microbiology, Gurukula Kangri (Deemed to Be University), Haridwar, 249404, India.ORCID http://orcid.org/0000-0003-2041-5521
Ashwani KumarDepartment of Pharmaceutical Science, Gurukula Kangri (Deemed to Be University), Haridwar, 249404, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Probiotic attributes of lactic acid bacteria isolated from goat and sheep milk samples were analysed by culturing them on an MRS agar medium. The most potential isolates, GMB24 and SMB16, were identified by biochemical tests which had ability to tolerate different concentrations of acid and bile and phenol resistance. They were further identified as Enterococcus faecium GMB24 and Enterococcus hirae SMB16 by 16S rRNA gene sequencing approach. The probiotic potential of the isolates GMB24 and SMB16 were recorded including antimicrobial activity against pathogenic bacteria viz., Escherichia coli (MTCC118), Staphylococcus aureus (MTCC7443), Pseudomonas aeruginosa (MTCC424), Listeria monocytogens (MTCC657) and Salmonella typhimurium (MTCC733), and antibiotic susceptibility test. The isolates SMB16 and GMB24 exhibited a higher zone of inhibition against P. aeruginosa (19.00 ± 0.57 mm) and S. aureus (25.66 ± 0.88 mm), respectively. The data from these experiments were used for the principal component analysis (PCA) to assess the survivability of the isolates under different factors. The heatmap generated in this study clustered the bacterial isolates based on their phenotype properties. Further, immunomodulating activities of these probiotic bacteria were tested on neutrophil adhesion test, haemagglutinating antibody titer and delayed-type hypersensitivity. Probiotic E. faecium GMB24 and E. hirae SMB16, at 10

Indexed as

Enterococcus faeciumProbioticsAnimalsEnterococcus hiraeGoatsMilkRNA, Ribosomal, 16SSheepStaphylococcus aureusRNA, Ribosomal, 16SEnterococcus faecium GMB24Enterococcus hirae SMB16Heatmap analysisImmunomodulatory activityPrincipal component analysisProbiotic bacteria

Identifiers

PMID36098848

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.