ArticleNature communications2022
A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging.
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Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64citing papers in PubMed
8.8field-weighted citation impact, top 1% of its field
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64 citing papers in PubMed, 105 citations in OpenAlex.
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- Hunger states modulate aggression via opposing dopamine pathways.Nature communications · 2026Article
- Red-Shifted Epac-Based FRET cAMP Sensors for All-Optical cAMP Control and Multiparameter Imaging.Cells · 2026Article
- Protocol for simultaneous in vivo two-photon imaging and locomotion quantification during olfactory stimulation and pharmacology in walking Drosophila.STAR protocols · 2026Article
- Cyclic AMP compartmentalization drives signal specificity to control vector colonization and mammalian host infection by American trypanosomes.PLoS pathogens · 2026Article
- A highly sensitive genetically encoded red cAMP sensor for multiplex imaging in vivo.Nature communications · 2026Article
- A versatile method for designing biosensors via regulatory domains of allosteric enzymes.Nature communications · 2026Article
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- A dendrite-resolved,bioRxiv : the preprint server for biology · 2026Article
- Rab23 GTPase and IFT43 regulate the trafficking of prostaglandin E receptor 4 to primary cilia.Communications biology · 2025Article
- Cyclic AMP compartmentalization drives signal specificity to control vector colonization and mammalian host infection by American trypanosomes.bioRxiv : the preprint server for biology · 2025Article
- The neural circuits and signalling pathways of opioid use disorder.Nature reviews. Neuroscience · 2025Review
- Two novel red-FRET ERK bosensors in the 670-720 nm range.Journal of biological engineering · 2025Article
- Differential dopaminergic modulation of the antennal lobe of Drosophila melanogaster.Communications biology · 2025Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
27 authors at 8 institutions in 2 countries.
Liang Wang *Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.ORCID 0000-0002-6907-3457
Chunling Wu *PKU-IDG-McGovern Institute for Brain Research, Beijing, 100871, China.
Wanling PengInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
Ziliang ZhouMolecular Imaging Center, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, 519000, China.ORCID 0000-0002-6801-4180
Jianzhi ZengPKU-IDG-McGovern Institute for Brain Research, Beijing, 100871, China.ORCID 0000-0002-5380-6281
Xuelin LiPKU-IDG-McGovern Institute for Brain Research, Beijing, 100871, China.
Yini YangPKU-IDG-McGovern Institute for Brain Research, Beijing, 100871, China.
Shuguang YuState Key Laboratory of Neuroscience, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.
Ye ZouDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, 66506, KS, USA.ORCID 0000-0002-3145-9661
Mian HuangDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, 66506, KS, USA.
Chang LiuInstitute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Yefei ChenBrain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Yi LiKey Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Panpan TiKey Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Wenfeng LiuResearch Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.ORCID 0000-0003-0747-1852
Yufeng GaoResearch Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Wei ZhengResearch Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.ORCID 0000-0001-6107-7261
Haining ZhongVollum Institute, Oregon Health and Science University, Portland, 97239, OR, USA.ORCID 0000-0002-7109-4724
Shangbang GaoKey Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.ORCID 0000-0001-5431-4628
Zhonghua LuBrain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Pei-Gen RenInstitute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
Ho Leung NgDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, 66506, KS, USA.ORCID 0000-0002-6415-1938
Jie HeState Key Laboratory of Neuroscience, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.ORCID 0000-0002-2539-2616
Shoudeng ChenMolecular Imaging Center, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, 519000, China.ORCID 0000-0002-7634-2141
Min XuInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China.ORCID 0000-0002-6936-7543
Yulong LiPKU-IDG-McGovern Institute for Brain Research, Beijing, 100871, China.ORCID 0000-0002-9166-9919
Jun ChuResearch Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. jun.chu@siat.ac.cn.ORCID 0000-0002-9925-5198
Chinese Academy of Sciences · CNMcGovern Institute for Brain Research · USShenzhen Institutes of Advanced Technology · CNKansas State University · USHuazhong University of Science and Technology · CNSun Yat-sen University · CNOregon Health & Science University · USShenzhen Institute of Neuroscience · CN
Funding
Neuromodulation in the striatumR01NS127013 · NINDS · OREGON HEALTH & SCIENCE UNIVERSITY · PI Haining Zhong · 2022 to 2026
$1.9MNINDS NIH HHS R01 NS127013
Abstract
cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular cAMP in intact organisms are largely unknown due to low sensitivity and/or brightness of current genetically encoded fluorescent cAMP indicators. Here, we report the development of the new circularly permuted GFP (cpGFP)-based cAMP indicator G-Flamp1, which exhibits a large fluorescence increase (a maximum ΔF/F
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Diagnostic ImagingSecond Messenger SystemsAnimalsColoring AgentsGreen Fluorescent ProteinsHEK293 CellsHumansMiceColoring AgentsGreen Fluorescent Proteins
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