ArticlePLoS genetics2022
Actin depolymerizing factor ADF7 inhibits actin bundling protein VILLIN1 to regulate root hair formation in response to osmotic stress in Arabidopsis.
Article in PLoS genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 18 citations in OpenAlex.
- Systematic Analysis of theLife (Basel, Switzerland) · 2026Article
- Review
- The RCAR12-CAP1-OST1 module controls ABA-mediated stomatal closure in Arabidopsis.PLoS genetics · 2026Article
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- The ADF4-RCAR12 module regulates ABA-mediated drought tolerance inFrontiers in plant science · 2026Article
- Functional Characterization ofInternational journal of molecular sciences · 2025Article
- Regulatory mechanisms of trichome and root hair development in Arabidopsis.Plant molecular biology · 2024Review
- Regulation of cytoskeleton dynamics and its interplay with force in plant cells.Biophysics reviews · 2024Review
- SPIRRIG is required for BRICK1 stability and salt stress induced root hair developmental plasticity in Arabidopsis.Stress biology · 2024Article
- MYB52 negatively regulates ADF9-meditated actin filament bundling in Arabidopsis pavement cell morphogenesis.Journal of integrative plant biology · 2024Article
- Dynamics of Actin Filaments Play an Important Role in Root Hair Growth under Low Potassium Stress inInternational journal of molecular sciences · 2024Article
- Evolution of the thermostability of actin-depolymerizing factors enhances the adaptation of pollen germination to high temperature.The Plant cell · 2024Article
- Genome-Wide Analysis ofCurrent issues in molecular biology · 2024Article
- Actin-depolymerizing factors 8 and 11 promote root hair elongation at high pH.Plant communications · 2024Article
- Genome-wide identification of actin-depolymerizing factor family genes in melon (Frontiers in plant science · 2024Article
- Genome-wide characterization and expression analysis of theFrontiers in plant science · 2024Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Phosphorylation of RhoGDI1, a Rho GDP dissociation inhibitor, regulates root hair development inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Research progress on the roles of actin-depolymerizing factor in plant stress responses.Frontiers in plant science · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin cytoskeleton is essential for root hair formation. However, the underlying molecular mechanisms of actin dynamics in root hair formation in response to abiotic stress are largely undiscovered. Here, genetic analysis showed that actin-depolymerizing protein ADF7 and actin-bundling protein VILLIN1 (VLN1) were positively and negatively involved in root hair formation of Arabidopsis respectively. Moreover, RT-qPCR, GUS staining, western blotting, and genetic analysis revealed that ADF7 played an important role in inhibiting the expression and function of VLN1 during root hair formation. Filament actin (F-actin) dynamics observation and actin pharmacological experiments indicated that ADF7-inhibited-VLN1 pathway led to the decline of F-actin bundling and thick bundle formation, as well as the increase of F-actin depolymerization and turnover to promote root hair formation. Furthermore, the F-actin dynamics mediated by ADF7-inhibited-VLN1 pathway was associated with the reactive oxygen species (ROS) accumulation in root hair formation. Finally, ADF7-inhibited-VLN1 pathway was critical for osmotic stress-induced root hair formation. Our work demonstrates that ADF7 inhibits VLN1 to regulate F-actin dynamics in root hair formation in response to osmotic stress, providing the novel evidence on the F-actin dynamics and their molecular mechanisms in root hair formation and in abiotic stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.