Evidence map›Paper›PMID 36094735›Full record

ArticleGenes & genomics2022

KDM3A promotes oral squamous cell carcinoma cell proliferation and invasion via H3K9me2 demethylation-activated DCLK1.

Lei Yang, Qiqiong Zhang, Qiuye Yang

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In one paragraph

Article in Genes & genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Lei Yang *Department of Prosthodontics, Daqing Oilfield General Hospital, No. 9 Zhongkang Street, Saertu District, Daqing City, 163001, Heilongjiang Province, China. yangleihlj@163.com.ORCID 0000-0001-9754-6592
Qiqiong Zhang *Department of Prosthodontics, Daqing Oilfield General Hospital, No. 9 Zhongkang Street, Saertu District, Daqing City, 163001, Heilongjiang Province, China.
Qiuye YangDepartment of Prosthodontics, Daqing Oilfield General Hospital, No. 9 Zhongkang Street, Saertu District, Daqing City, 163001, Heilongjiang Province, China.
Daqing Oilfield General Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundOral squamous cell carcinoma (OSCC) is a frequently-diagnosed malignancy with high potential for proliferation and invasion. Histone methylation is known as a crucial mechanism that regulates pathological processes in various cancers, including OSCC.

objectiveThis study sought to delve into the molecular mechanism of lysine demethylase 3 A (KDM3A) in OSCC cell proliferation and invasion.

methodsExpression levels of KDM3A, lysin-9 of di-methylated histone H3 (H3K9me2), and doublecortin-like kinase 1 (DCLK1) in cells were determined by reverse-transcription quantitative polymerase chain reaction or Western blot analysis. Cell proliferation and invasion were evaluated by cell counting kit-8, colony formation, and Transwell assays. The enrichment of KDM3A and H3K9me2 on the DCLK1 promoter was determined by chromatin immunoprecipitation assay. The functional rescue experiment was performed with DCLK1 overexpression vector and si-KDM3A in CAL-27 and SCC-9 cells.

resultsKDM3A was elevated in OSCC cells. KDM3A knockdown suppressed OSCC proliferation and invasion, along with increased H3K9me2 level in OSCC cells. KDM3A and H3K9me2 were enriched on the DCLK1 promoter and inhibiting H3K9me2 improved DCLK1 expression levels. DCLK1 overexpression neutralized the inhibition of KDM3A knockdown on OSCC proliferation and invasion.

conclusionsKDM3A facilitated OSCC proliferation and invasion by eliminating H3K9me2 to upregulate DCLK1 expression levels.

Indexed as

Doublecortin-Like KinasesJumonji Domain-Containing Histone DemethylasesMouth NeoplasmsSquamous Cell Carcinoma of Head and NeckCell ProliferationDemethylationHistonesHumansIntracellular Signaling Peptides and ProteinsLysineProtein Serine-Threonine KinasesDCLK1 protein, humanDoublecortin-Like KinasesHistonesIntracellular Signaling Peptides and ProteinsJumonji Domain-Containing Histone DemethylasesKDM3A protein, humanLysineProtein Serine-Threonine KinasesDCLK1H3K9me2InvasionKDM3AOral squamous cell carcinomaProliferation

Identifiers

PMID36094735
OpenAlexW4295272941

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.