ArticleArchives of microbiology2022
A circular cell culture system using microalgae and mammalian myoblasts for the production of sustainable cultured meat.
Article in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 52 citations in OpenAlex.
- Algal and Cyanobacterial Hydrolysates as Serum-Reducing Supplements Across Mammalian, Avian and Fish Cells with Identification of Bioactive Chromopeptides.Foods (Basel, Switzerland) · 2026Article
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
- Valorization of Hemp, Shrimp and Blue Crab Co-Products as Novel Culture Media Ingredients to Improve Protein Quality and Antioxidant Capacity of Cultured Meat in Cell-Based Food Applications.Foods (Basel, Switzerland) · 2026Article
- Animal-derived components in cultivated meat research and their alternatives.NPJ science of food · 2025Review
- Crop cultivation without nitrogen fertiliser using nitrogen-fixing cyanobacterial extracts for low environmental impact.Scientific reports · 2025Article
- Exploring Sustainable Future Protein Sources.Food science of animal resources · 2025Review
- Development and optimization of an ammonia removal strategy for sustainable recycling of cell culture spent media in cultivated meat production: from concept to implementation.Frontiers in bioengineering and biotechnology · 2025Article
- Exploring cost reduction strategies for serum free media development.NPJ science of food · 2024Review
- Screening Algal and Cyanobacterial Extracts to Identify Potential Substitutes for Fetal Bovine Serum in Cellular Meat Cultivation.Foods (Basel, Switzerland) · 2024Article
- A serum-free culture medium production system by co-culture combining growth factor-secreting cells and L-lactate-assimilating cyanobacteria for sustainable cultured meat production.Scientific reports · 2024Article
- Cultured meat in the European Union: Legislative context and food safety issues.Current research in food science · 2024Review
- Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids.Archives of microbiology · 2023Article
- L-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous L-lactate dehydrogenase.Scientific reports · 2023Article
- Nutrient recovery in cultured meat systems: Impacts on cost and sustainability metrics.Frontiers in nutrition · 2023Article
- Analysis of the economic viability and environmental impacts of a conceptual process model for the recovery of lactic acid from spent media in cultivated meat production.Biotechnology progressArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For sustainable production of cultured meat, we propose a novel circular cell culture (CCC) system in which microalgae are used as nutrient supply for the mammalian cell culture and as a waste-medium recycler. Chlorococcum littorale, RL34 hepatocytes, and C2C12 myoblasts were used as cell sources for microalgae, growth factor-producing cells, and muscle cells, respectively. In the first cycle, C2C12 cells were amplified 4.0-fold after 48 h of culture in an RL34 cell-conditioned medium. In the second cycle, C2C12 cells were cultured in the C. littorale culture waste medium to which the C. littorale-derived nutrients were added. The proliferation rates of C. littorale and C2C12 and the nutrient extraction efficiency from C. littorale were the same in the first and second cycles. Therefore, this CCC system, which works without additional grain-derived nutrients and animal sera, will help drastically reduce environmental load, resource/energy consumption, and costs in future cultured meat production.
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