Evidence map›Paper›PMID 36094301›Full record

ArticleMicrobiology spectrum2022

A Novel Cryptic Clostridial Peptide That Kills Bacteria by a Cell Membrane Permeabilization Mechanism.

Monika Szadkowska, Michal Olewniczak, Anna Kloska, Elzbieta Jankowska, Malgorzata Kapusta, Bartosz Rybak, Dariusz Wyrzykowski, Wioletta Zmudzinska, Artur Gieldon, Aleksandra Kocot and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Monika Szadkowska *Laboratory of Extremophiles Biology, Department of Microbiology, Faculty of Biology, University of Gdansk, Gdansk, Poland.
Michal Olewniczak *Department of Physical Chemistry, Gdansk University of Technology, Gdansk, Poland.
Anna KloskaDepartment of Medical Biology and Genetics, Faculty of Biology, University of Gdansk, Gdansk, Poland.
Elzbieta JankowskaDepartment of Biomedical Chemistry, Faculty of Chemistry, University of Gdansk, Gdansk, Poland.
Malgorzata KapustaDepartment of Plant Cytology and Embryology, Faculty of Biology, University of Gdansk, Gdansk, Poland.
Bartosz RybakDepartment of Environmental Toxicology, Faculty of Health Sciences with Institute of Maritime and Tropical Medicine, Medical University of Gdansk, Gdansk, Poland.
Dariusz WyrzykowskiDepartment of General and Inorganic Chemistry, Faculty of Chemistry, University of Gdansk, Gdansk, Poland.
Wioletta ZmudzinskaLaboratory of Biopolymer Structure, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Gdansk, Poland.
Artur GieldonLaboratory of Simulation of Polymers, Department of Theoretical Chemistry, Faculty of Chemistry, University of Gdansk, Gdansk, Poland.ORCID 0000-0003-0415-9214
Aleksandra KocotLaboratory of Extremophiles Biology, Department of Microbiology, Faculty of Biology, University of Gdansk, Gdansk, Poland.
Anna-Karina KaczorowskaCollection of Plasmids and Microorganisms, Faculty of Biology, University of Gdansk, Gdansk, Poland.
Lukasz NierzwickiDepartment of Physical Chemistry, Gdansk University of Technology, Gdansk, Poland.
Joanna MakowskaDepartment of General and Inorganic Chemistry, Faculty of Chemistry, University of Gdansk, Gdansk, Poland.
Tadeusz KaczorowskiLaboratory of Extremophiles Biology, Department of Microbiology, Faculty of Biology, University of Gdansk, Gdansk, Poland.
Magdalena PlotkaLaboratory of Extremophiles Biology, Department of Microbiology, Faculty of Biology, University of Gdansk, Gdansk, Poland.ORCID 0000-0002-2871-8060
University of Gdańsk · PLGdańsk Medical University · PLGdańsk University of Technology · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This work reports detailed characteristics of the antimicrobial peptide Intestinalin (P30), which is derived from the LysC enzyme of Clostridium intestinale strain URNW. The peptide shows a broader antibacterial spectrum than the parental enzyme, showing potent antimicrobial activity against clinical strains of Gram-positive staphylococci and Gram-negative pathogens and causing between 3.04 ± 0.12 log kill for Pseudomonas aeruginosa PAO1 and 7.10 ± 0.05 log kill for multidrug-resistant Acinetobacter baumannii KPD 581 at a 5 μM concentration. Moreover, Intestinalin (P30) prevents biofilm formation and destroys 24-h and 72-h biofilms formed by Acinetobacter baumannii CRAB KPD 205 (reduction levels of 4.28 and 2.62 log CFU/mL, respectively). The activity of Intestinalin is combined with both no cytotoxicity and little hemolytic effect against mammalian cells. The nuclear magnetic resonance and molecular dynamics (MD) data show a high tendency of Intestinalin to interact with the bacterial phospholipid cell membrane. Although positively charged, Intestinalin resides in the membrane and aggregates into small oligomers. Negatively charged phospholipids stabilize peptide oligomers to form water- and ion-permeable pores, disrupting the integrity of bacterial cell membranes. Experimental data showed that Intestinalin interacts with negatively charged lipoteichoic acid (log

Indexed as

Anti-Bacterial AgentsBacteriaPeptidesAcinetobacter baumanniiAdenosine TriphosphateAmino AcidsAnimalsCell MembraneMammalsMicrobial Sensitivity TestsPhospholipidsWaterAdenosine TriphosphateAmino AcidsAnti-Bacterial AgentsPeptidesPhospholipidsWaterantibacterial peptidesendolysinsmembrane potentialmolecular dynamicstoroidal pore model

Identifiers

PMID36094301
PMCPMC9602519
OpenAlexW4295279495

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.