Evidence map›Paper›PMID 36094214›Full record

ArticleApplied and environmental microbiology2022

Effects of Nutrient Level and Growth Rate on the Conjugation Process That Transfers Mobile Antibiotic Resistance Genes in Continuous Cultures.

Mohammadreza Shafieifini, Yuepeng Sun, Zachery R Staley, Jean-Jack Riethoven, Xu Li

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Article
  3. Seaweed exposure modulatesMicrobiology (Reading, England) · 2025
    Article
  4. The group AInfection and immunity · 2025
    Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Mohammadreza ShafieifiniDepartment of Civil and Environmental Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Yuepeng SunDepartment of Civil and Environmental Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Zachery R StaleyDepartment of Civil and Environmental Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Jean-Jack RiethovenNebraska Center for Biotechnology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
Xu LiDepartment of Civil and Environmental Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.ORCID 0000-0002-1006-3027
University of Nebraska–Lincoln · US

Funding

UNMC Structural Biology CoreP20GM103427 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Heather Colleen Jensen-Smith · 2012 to 2026
$59.2M
UNMC/EPPLEY CANCER CENTER SUPPORT GRANTP30CA036727 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI Apar Kishor Ganti · 1985 to 2026
$55.0M
UNMC Histology/Imaging and Creighton University Imaging Core FacilityP30GM110768 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI SMITH, SHELLEY D · 2014 to 2018
$5.1M
NCI NIH HHS P30 CA036727NIGMS NIH HHS P20 GM103427NIGMS NIH HHS P30 GM110768
6 · The paper itself

Abstract

Bacteria in the effluent of wastewater treatment plants (WWTPs) can transfer antibiotic resistance genes (ARGs) to the bacteria in receiving water through conjugation; however, there is a lack of quantitative assessment of this phenomenon in continuous cultures. Our objective was to determine the effects of background nutrient levels in river water column and growth rates of bacteria on the conjugation frequency of ARGs from effluent bacteria to river bacteria, as well as on the resulting resistance level (i.e., MICs) of the river bacteria. Chemostats were employed to simulate the discharge points of WWTPs into rivers, where effluent bacteria (donor cells) meet river bacteria (recipient cells). Both donor and recipient cells were Escherichia coli cells, and the donor cells were constructed by filter mating with bacteria in the effluent of a local WWTP. Results showed that higher bacterial growth rate (0.45 h

Indexed as

Anti-Bacterial AgentsWastewaterBacteriaDrug Resistance, MicrobialEcosystemEscherichia coliGenes, BacterialHumansNutrientsTetracyclineWaterAnti-Bacterial AgentsNutrientsTetracyclineWastewaterWaterchemostatconjugationE. coligrowth ratenutrient leveltetracycline resistance

Identifiers

PMID36094214
PMCPMC9552606
OpenAlexW4295280949

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.