ArticleeLife2022
Live-cell imaging in human colonic monolayers reveals ERK waves limit the stem cell compartment to maintain epithelial homeostasis.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Patient-Derived Organoids as a Model to Understand Tumor Microenvironment-Driven Nano-Bio Interactions - A Framework Towards Improved Nanomedicine Translation.bioRxiv : the preprint server for biology · 2026Article
- High-throughput quantitation of pathogen-induced calcium signals captured through live-cell fluorescence microscopy.Cell calcium · 2026Article
- Preservation of Human Colonic Stem Cells Requires an ERK Dynamics Checkpoint Mediated by AKT.bioRxiv : the preprint server for biology · 2026Article
- Wnt signaling is modulated by the buffer-like properties of cadherins.Molecular biology of the cell · 2026Article
- Serial Imaging of Tumour and microEnvironment (SITE) platform for live-cellbioRxiv : the preprint server for biology · 2025Article
- Enteroendocrine cell differentiation: Implications for human disease.Molecular and cellular endocrinology · 2025Review
- Dapagliflozin ameliorates intestinal stem cell aging by regulating the MAPK signaling pathway inFrontiers in cell and developmental biology · 2025Article
- Reduction of endocytosis and EGFR signaling is associated with the switch from isolated to clustered apoptosis during epithelial tissue remodeling in Drosophila.PLoS biology · 2024Article
- Oncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation.Nature cell biology · 2024Article
- Formation of recurring transient CaProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers.Tissue barriers · 2024Article
- Quantification of collective signalling in time-lapse microscopy images.Methods in microscopy · 2024Article
- Attenuation of Chronic Inflammation in Intestinal Organoids with Graphene Oxide-Mediated Tumor Necrosis Factor-α_Small Interfering RNA Delivery.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Review
- Collective signalling drives rapid jumping between cell states.Development (Cambridge, England) · 2023Article
- Detecting host responses to microbial stimulation using primary epithelial organoids.Gut microbes · 2023Article
- Automatic detection of spatio-temporal signaling patterns in cell collectives.The Journal of cell biology · 2023Article
- FOXO nuclear shuttling dynamics are stimulus-dependent and correspond with cell fate.Molecular biology of the cell · 2023Article
- Article
- Multi-Aperture Transformers for 3D (MAT3D) Segmentation of Clinical and Microscopic Images.IEEE Winter Conference on Applications of Computer Vision. IEEE Winter Conference on Applications of Computer VisionArticle
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Authors and funding
11 authors.
Funding
Abstract
The establishment and maintenance of different cellular compartments in tissues is a universal requirement across all metazoans. Maintaining the correct ratio of cell types in time and space allows tissues to form patterned compartments and perform complex functions. Patterning is especially evident in the human colon, where tissue homeostasis is maintained by stem cells in crypt structures that balance proliferation and differentiation. Here, we developed a human 2D patient derived organoid screening platform to study tissue patterning and kinase pathway dynamics in single cells. Using this system, we discovered that waves of ERK signaling induced by apoptotic cells play a critical role in maintaining tissue patterning and homeostasis. If ERK is activated acutely across all cells instead of in wave-like patterns, then tissue patterning and stem cells are lost. Conversely, if ERK activity is inhibited, then stem cells become unrestricted and expand dramatically. This work demonstrates that the colonic epithelium requires coordinated ERK signaling dynamics to maintain patterning and tissue homeostasis. Our work reveals how ERK can antagonize stem cells while supporting cell replacement and the function of the gut.
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