Evidence map›Paper›PMID 36092821›Full record

ArticleSTAR protocols2022

Live-cell metabolic analysis of oligodendroglia isolated from postnatal mouse brain and spinal cord.

Luipa Khandker, Teresa L Wood

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. mTORC2 Loss in Oligodendrocyte Progenitor Cells Results in Regional Hypomyelination in the Central Nervous System.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Luipa KhandkerDepartment of Pharmacology, Physiology & Neuroscience, New Jersey Medical School, Rutgers University, Newark, NJ 07103, USA. Electronic address: luipa.khandker@gmail.com.
Teresa L WoodDepartment of Pharmacology, Physiology & Neuroscience, New Jersey Medical School, Rutgers University, Newark, NJ 07103, USA. Electronic address: terri.wood@rutgers.edu.
Rutgers, The State University of New Jersey · US

Funding

The role of mTOR signaling in oligodendrocyte differentiation and CNS myelinationR37NS082203 · NINDS · UNIVERSITY OF COLORADO DENVER · PI MACKLIN, WENDY B, WOOD, TERESA L · 2017 to 2023
$6.0M
The role of mTOR signaling in oligodendrocyte differentiation and CNS myelinationR01NS082203 · NINDS · UNIVERSITY OF COLORADO DENVER · PI MACKLIN, WENDY B, WOOD, TERESA L · 2012 to 2016
$3.3M
NINDS NIH HHS R01 NS082203NINDS NIH HHS R37 NS082203
6 · The paper itself

Abstract

This protocol describes isolation and live-cell metabolic analysis of O4+ oligodendroglia from brain and spinal cord of postnatal mice. We have optimized existing protocols for O4+ isolation from neonatal brain and expanded the protocol to include isolation of highly viable oligodendroglia from spinal cords of postnatal mice up to 18 days of age. Isolated oligodendroglia can be used in multiple downstream analyses, and here we describe an optimized real-time metabolic assay using Agilent Seahorse Analyzer to measure mitochondrial respiration. For complete details on the use and execution of this protocol, please refer to Khandker et al. (2022).

Indexed as

OligodendrogliaSpinal CordAnimalsBrainMiceMitochondriaOxygen ConsumptionCell-based assaysCell isolationMetabolismNeuroscience

Identifiers

PMID36092821
PMCPMC9449657
OpenAlexW4293328355

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.