ArticleInorganic chemistry frontiers2022
Lanmodulin peptides - unravelling the binding of the EF-Hand loop sequences stripped from the structural corset.
Article in Inorganic chemistry frontiers, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Machine Learning-Based Development of Gadolinium Binding Peptides.NMR in biomedicine · 2026Article
- Article
- A family portrait of lanmodulin selectivity for enhanced rare-earth separations.Nature chemical biology · 2026Article
- A lanmodulin homologous protein, from Mesorhizobium qingshengii J19, involved in yttrium immobilization.Scientific reports · 2026Article
- Urban Biomining of Rare Earth Elements: Current Status and Future Opportunities.ACS environmental Au · 2026Review
- A Luminescence-Based Screening Platform for Lanthanide-Binding Peptides and Proteins.ACS chemical biology · 2025Article
- Machine learning-based development of Gadolinium binding peptides.bioRxiv : the preprint server for biology · 2025Article
- Reversing Lanmodulin's Metal-Binding Sequence in Short Peptides Surprisingly Increases the Lanthanide Affinity.Angewandte Chemie (International ed. in English) · 2025Article
- X-ray absorption spectroscopy of lanmodulin-derived peptides bound to rare earth elements.Journal of synchrotron radiation · 2025Article
- Mining peptides for mining solutions: evaluation of calcium-binding peptides for rare earth element separations.Chemical science · 2025Article
- The Role of Asparagine as a Gatekeeper Residue in the Selective Binding of Rare Earth Elements by Lanthanide-Binding Peptides.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- A novel protein for bioremediation of gadolinium waste.Protein science : a publication of the Protein Society · 2025Article
- The Soil-Plant Continuity of Rare Earth Elements: Insights into an Enigmatic Class of Xenobiotics and Their Interactions with Plant Structures and Processes.Journal of xenobiotics · 2025Review
- Lanthanide-Controlled Protein Switches: Development and In Vitro and In Vivo Applications.Angewandte Chemie (International ed. in English) · 2025Article
- Computationally derived structural insights into Rare Earth selectivity in lanmodulin and its variants.Computational and structural biotechnology journal · 2025Article
- Lanthanide binding peptide surfactants at air-aqueous interfaces for interfacial separation of rare earth elements.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A novel in-silico model explores LanM homologs among Hyphomicrobium spp.Communications biology · 2024Article
- A Review of the Occurrence and Recovery of Rare Earth Elements from Electronic Waste.Molecules (Basel, Switzerland) · 2024Review
- Rare earth metallic elements in plants: assessing benefits, risks and mitigating strategies.Plant cell reports · 2024Review
- Rare earth elements in biology: From biochemical curiosity to solutions for extractive industries.Microbial biotechnology · 2024Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lanmodulin (LanM), a naturally lanthanide (Ln)-binding protein with a remarkable selectivity for Lns over Ca(ii) and affinities in the picomolar range, is an attractive target to address challenges in Ln separation. Why LanM has such a high selectivity is currently not entirely understood; both specific amino acid sequences of the EF-Hand loops and cooperativity effects have been suggested. Here, we removed the effect of cooperativity and synthesised all four 12-amino acid EF-Hand loop peptides, and investigated their affinity for two Lns (Eu(iii) and Tb(iii)), the actinide Cm(iii) and Ca(ii). Using isothermal titration calorimetry and time-resolved laser fluorescence spectroscopy (TRLFS) combined with parallel factor analysis, we show that the four short peptides behave very similarly, having affinities in the micromolar range for Eu(iii) and Tb(iii). Ca(ii) was shown not to bind to the peptides, which was verified with circular dichroism spectroscopy. This technique also revealed an increase in structural organisation upon Eu(iii) addition, which was supported by molecular dynamics simulations. Lastly, we put Eu(iii) and Cm(iii) in direct competition using TRLFS. Remarkably, a slightly higher affinity for Cm(iii) was found. Our results demonstrate that the picomolar affinities in LanM are largely an effect of pre-structuring and therefore a reduction of flexibility in combination with cooperative effects, and that all EF-Hand loops possess similar affinities when detached from the protein backbone, albeit still retaining the high selectivity for lanthanides and actinides over calcium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.