ArticlePlant communications2023
CRISPR/dCas-mediated gene activation toolkit development and its application for parthenogenesis induction in maize.
Article in Plant communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- CitRWP directly activates CsDAZ3 to promote somatic embryogenesis in Citrus.The New phytologist · 2026Article
- Development of a low-coverage whole genome sequencing screen for apomixis using a diverse set of Malus germplasm.PLoS genetics · 2026Article
- Basis of apomixis in flowering plants - state of the art and research perspectives.Plant reproduction · 2026Review
- How Apomictic and Sexual Cenchrus Species Can Help Elucidate the Mechanisms Leading to Apospory.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- The Potential of NGTs to Overcome Constraints in Plant Breeding and Their Regulatory Implications.International journal of molecular sciences · 2025Review
- Article
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- Revolutionizing molecular plant breeding through genetic engineering of in vivo haploid induction genes.Molecular biology reports · 2025Review
- Epigenome Engineering Using dCas Systems for Biomedical Applications and Biotechnology: Current Achievements, Opportunities and Challenges.International journal of molecular sciences · 2025Review
- Integrating Genetic Diversity and Agronomic Innovations for Climate-Resilient Maize Systems.Plants (Basel, Switzerland) · 2025Review
- Advances in Genome Editing Through Haploid Induction Systems.International journal of molecular sciences · 2025Review
- Unlocking Nature's Shield: The Promising Potential of CRISPRa in Amplifying Antimicrobial Peptide Expression in Common Bean (ACS omega · 2025Article
- To infinity and beyond: recent progress, bottlenecks, and potential of clonal seeds by apomixis.The Plant journal : for cell and molecular biology · 2025Review
- Perspectives of Genome Editing Mediated Haploid Inducer Systems in Legumes.International journal of molecular sciences · 2025Review
- Advances in doubled haploid technology for rice breeding: mechanisms, applications, and future perspectives.Frontiers in plant science · 2025Review
- Widespread application of apomixis in agriculture requires further study of natural apomicts.iScience · 2024Review
- Haploid identification in maize.Frontiers in plant science · 2024Review
- Haploid induction: an overview of parental factor manipulation during seed formation.Frontiers in plant science · 2024Review
- CRISPR/Cas Technology Revolutionizes Crop Breeding.Plants (Basel, Switzerland) · 2023Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems can be engineered as programmable transcription factors to either activate (CRISPRa) or inhibit transcription. Apomixis is extremely valuable for the seed industry in breeding clonal seeds with pure genetic backgrounds. We report here a CRISPR/dCas9-based toolkit equipped with dCas9-VP64 and MS2-p65-HSF1 effectors that may specifically target genes with high activation capability. We explored the application of in vivo CRISPRa targeting of maize BABY BOOM2 (ZmBBM2), acting as a fertilization checkpoint, as a means to engineer parthenogenesis. We detected ZmBBM2 transcripts only in egg cells but not in other maternal gametic cells. Activation of ZmBBM2 in egg cells in vivo caused maternal cell-autonomous parthenogenesis to produce haploid seeds. Our work provides a highly specific gene-activation CRISPRa technology for target cells and verifies its application for parthenogenesis induction in maize.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.