Evidence map›Paper›PMID 36089749›Full record

ArticleJournal of medical virology2023

Rapid and reliable inactivation protocols for the diagnostics of emerging viruses: The example of SARS-CoV-2 and monkeypox virus.

Grégory Quéromès, Emilie Frobert, Maude Bouscambert-Duchamp, Antoine Oblette, Martine Valette, Geneviève Billaud, Vanessa Escuret, Bruno Lina, Florence Morfin, Alexandre Gaymard

Open access · hybridAbstract read
In one paragraph

Article in Journal of medical virology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. The Biosafety Research Road Map: The Search for Evidence to Support Practices in the Laboratory-SARS-CoV-2.Applied biosafety : journal of the American Biological Safety Association · 2023
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Grégory QuéromèsCentre International de Recherche en Infectiologie (CIRI), Team VirPatH, Université de Lyon, Inserm, U1111, CNRS, UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Lyon, France.ORCID 0000-0001-8176-3955
Emilie FrobertCentre International de Recherche en Infectiologie (CIRI), Team VirPatH, Université de Lyon, Inserm, U1111, CNRS, UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Lyon, France.
Maude Bouscambert-DuchampLaboratoire de Virologie, Institut des Agents Infectieux, Hospices Civils de Lyon, Lyon, France.
Antoine ObletteLaboratoire de Virologie, Institut des Agents Infectieux, Hospices Civils de Lyon, Lyon, France.
Martine ValetteCentre International de Recherche en Infectiologie (CIRI), Team VirPatH, Université de Lyon, Inserm, U1111, CNRS, UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Lyon, France.
Geneviève BillaudLaboratoire de Virologie, Institut des Agents Infectieux, Hospices Civils de Lyon, Lyon, France.
Vanessa EscuretCentre International de Recherche en Infectiologie (CIRI), Team VirPatH, Université de Lyon, Inserm, U1111, CNRS, UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Lyon, France.
Bruno LinaCentre International de Recherche en Infectiologie (CIRI), Team VirPatH, Université de Lyon, Inserm, U1111, CNRS, UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Lyon, France.
Florence MorfinCentre International de Recherche en Infectiologie (CIRI), Team VirPatH, Université de Lyon, Inserm, U1111, CNRS, UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Lyon, France.
Alexandre GaymardCentre International de Recherche en Infectiologie (CIRI), Team VirPatH, Université de Lyon, Inserm, U1111, CNRS, UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, Lyon, France.
Université Claude Bernard Lyon 1 · FRHospices Civils de Lyon · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The emergence and sustained transmission of novel pathogens are exerting an increasing demand on the diagnostics sector worldwide, as seen with the ongoing severe acute respiratory coronavirus 2 (SARS-CoV-2) pandemic and the more recent public health concern of monkeypox virus (MPXV) since May 2022. Appropriate and reliable viral inactivation measures are needed to ensure the safety of personnel handling these infectious samples. In the present study, seven commercialized diagnosis buffers, heat (56°C and 60°C), and sodium dodecyl sulfate detergent (2.0%, 1.0%, and 0.5% final concentrations) were tested against infectious SARS-CoV-2 and MPXV culture isolates on Vero cell culture. Cytopathic effects were observed up to 7 days postinoculation and viral load evolution was measured by semiquantitative polymerase chain reaction. The World Health Organization recommends an infectious titer reduction of at least 4 log

Indexed as

COVID-19SARS-CoV-2AnimalsChlorocebus aethiopsCOVID-19 TestingHumansMonkeypox virusVero CellsVirus Inactivationemerging pathogeninactivationmonkeypox virusrisk managementSARS-CoV-2

Identifiers

PMID36089749
PMCPMC9538322
OpenAlexW4296523883

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.