ArticleJournal of structural biology2022
Multi-curve fitting and tubulin-lattice signal removal for structure determination of large microtubule-based motors.
Article in Journal of structural biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Activation and regulation of the dynein-dynactin-NuMA complex.Nature chemical biology · 2026Article
- Article
- Molecular structure of the ESCRT-III-based archaeal CdvAB cell division machinery.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The mechanochemical cycle of reactive full-length human dynein 1.Nature structural & molecular biology · 2025Article
- Asgard archaea reveal the conserved principles of ESCRT-III membrane remodeling.Science advances · 2025Article
- Review
- Molecular basis for the assembly of the dynein transport machinery on microtubules.bioRxiv : the preprint server for biology · 2024Article
- In situ structural determination of cyanobacterial phycobilisome-PSII supercomplex by STAgSPA strategy.Nature communications · 2024Article
- Article
- High-resolutionbioRxiv : the preprint server for biology · 2024Article
- Structural determination and modeling of ciliary microtubules.Acta crystallographica. Section D, Structural biology · 2024Review
- Molecular mechanism of dynein-dynactin complex assembly by LIS1.Science (New York, N.Y.) · 2024Article
- Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes.The Journal of cell biology · 2023Article
- High-Resolution Structural Analysis of Dyneins by Cryo-electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Cryo-EM structure of an active central apparatus.Nature structural & molecular biology · 2022Article
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Authors and funding
3 authors.
Funding
Abstract
Revealing high-resolution structures of microtubule-associated proteins (MAPs) is critical for understanding their fundamental roles in various cellular activities, such as cell motility and intracellular cargo transport. Nevertheless, large flexible molecular motors that dynamically bind and release microtubule networks are challenging for cryo-electron microscopy (cryo-EM). Traditional structure determination of MAPs bound to microtubules needs alignment information from the reconstruction of microtubules, which cannot be readily applied to large MAPs without a fixed binding pattern. Here, we developed a comprehensive approach to estimate the microtubule networks (multi-curve fitting), model the tubulin-lattice signals, and remove them (tubulin-lattice subtraction) from the raw cryo-EM micrographs. The approach does not require an ordered binding pattern of MAPs on microtubules, nor does it need a reconstruction of the microtubules. We demonstrated the capability of our approach using the reconstituted outer-arm dynein (OAD) bound to microtubule doublets. The tubulin-lattice subtraction improves the OAD alignment, thus leading to high-resolution reconstructions. In addition, the multi-curve fitting approach provides an accurate automatic alternative method to pick or segment filaments in 2D images and potentially in 3D tomograms. The accuracy of our approach has been demonstrated by using several other biological filaments. Our work provides a new tool to determine high-resolution structures of large MAPs bound to curved microtubule networks.
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