Evidence map›Paper›PMID 36088528›Full record

ArticleJournal of biomedical optics2022

Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators.

Ruiying Zhang, Lei S Li, Bin Rao, Haoyang Rong, Min-Yu Sun, Junjie Yao, Ruimin Chen, Qifa Zhou, Steven Mennerick, Baranidharan Raman and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical optics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Ruiying ZhangWashington University in Saint Louis, Department of Biomedical Engineering, Saint Louis, Missouri, United States, United States.
Lei S LiCalifornia Institute of Technology, Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, Pasadena, California, United States, United States.
Bin RaoWashington University in Saint Louis, Department of Biomedical Engineering, Saint Louis, Missouri, United States, United States.
Haoyang RongWashington University in Saint Louis, Department of Biomedical Engineering, Saint Louis, Missouri, United States, United States.
Min-Yu SunWashington University School of Medicine, Department of Psychiatry, Saint Louis, Missouri, United States, United States.
Junjie YaoWashington University in Saint Louis, Department of Biomedical Engineering, Saint Louis, Missouri, United States, United States.
Ruimin ChenUniversity of Southern California, Department of Biomedical Engineering, Los Angeles, California, United States, United States.
Qifa ZhouUniversity of Southern California, Department of Biomedical Engineering, Los Angeles, California, United States, United States.
Steven MennerickWashington University School of Medicine, Department of Psychiatry, Saint Louis, Missouri, United States, United States.
Baranidharan RamanWashington University in Saint Louis, Department of Biomedical Engineering, Saint Louis, Missouri, United States, United States.
Lihong V WangCalifornia Institute of Technology, Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, Pasadena, California, United States, United States.
Washington University in St. Louis · USCalifornia Institute of Technology · USUniversity of Southern California · US

Funding

Deep brain photoacoustic tomography at single-neuron resolution using arrays of photonic emitters and high-frequency ultrasound transducersU01NS099717 · NINDS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI ROUKES, MICHAEL L, SHEPARD, KENNETH L · 2016 to 2018
$3.0M
Smart Laser Treatment of Port-Wine Stain in ChildrenK99AR062530 · NIAMS · WASHINGTON UNIVERSITY · PI RAO, BIN · 2012 to 2013
$172k
NIAMS NIH HHS K99 AR062530NINDS NIH HHS U01 NS099717
6 · The paper itself

Abstract

significanceOptical imaging of responses in fluorescently labeled neurons has progressed significantly in recent years. However, there is still a need to monitor neural activities at divergent spatial scales and at depths beyond the optical diffusion limit.

aimTo meet these needs, we aim to develop multiscale photoacoustic tomography (PAT) to image neural activities across spatial scales with a genetically encoded calcium indicator GCaMP. APPROACH: First, using photoacoustic microscopy, we show that depth-resolved GCaMP signals can be monitored in vivo from a fly brain in response to odor stimulation without depth scanning and even with the cuticle intact. In vivo monitoring of GCaMP signals was also demonstrated in mouse brains. Next, using photoacoustic computed tomography, we imaged neural responses of a mouse brain slice at depths beyond the optical diffusion limit.

resultsWe provide the first unambiguous demonstration that multiscale PAT can be used to record neural activities in transgenic flies and mice with select neurons expressing GCaMP.

conclusionsOur results indicate that the combination of multiscale PAT and fluorescent neural activity indicators provides a methodology for imaging targeted neurons at various scales.

Indexed as

CalciumTomography, X-Ray ComputedAnimalsBrainMiceNeuronsOptical ImagingCalciumcalcium indicatorsGCaMPneural imagingphotoacoustic tomography

Identifiers

PMID36088528
PMCPMC9463545
OpenAlexW4295095126

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.