ArticleCommunications biology2022
Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 73 citations in OpenAlex.
- A Comprehensive Review of Fucosylated Human Milk Oligosaccharides: Biological Functions, Production Technologies, and Safety Regulation With Commercial Translation in Infant Nutrition.Comprehensive reviews in food science and food safety · 2026Review
- Polyphenol oxidase depletion in Nicotiana benthamiana enhances recombinant protein purification and preserves native protein integrity.The New phytologist · 2026Article
- Rapid reconstitution and kinase-controlled regulation of algal pyrenoid condensates in engineeredbioRxiv : the preprint server for biology · 2026Article
- Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR.Synthetic and systems biotechnology · 2026Article
- Salicylic Acid-Induced Elicitation of Nepetalactone and Rosmarinic Acid Biosynthesis in Naked Catmint (International journal of molecular sciences · 2026Article
- Heterogeneity of iridoid biosynthesis in catmints: Molecular background in a phylogenetic context.Journal of integrative plant biology · 2026Article
- Complex engineering of Solanum alkaloids structural diversity in Nicotiana benthamiana.Plant biotechnology journal · 2026Article
- Metabolic engineering inaBIOTECH · 2025Review
- The missing link in the iridoid puzzle.Nature plants · 2025Article
- Discovery of iridoid cyclase completes the iridoid pathway in asterids.Nature plants · 2025Article
- Glyceollin biosynthesis in a plant chassis engineered for isoflavone production.Nature chemical biology · 2025Article
- A chromosome-level Mitragyna parvifolia genome unveils spirooxindole alkaloid diversification and mitraphylline biosynthesis.The Plant cell · 2025Article
- Phylogenomics and metabolic engineering reveal a conserved gene cluster in Solanaceae plants for withanolide biosynthesis.Nature communications · 2025Article
- Elucidating the enzyme network driving Amaryllidaceae alkaloids biosynthesis in Leucojum aestivum.Plant biotechnology journal · 2025Article
- Navigating the challenges of engineering composite specialized metabolite pathways in plants.The Plant journal : for cell and molecular biology · 2025Review
- Plant synthetic biology: from knowledge to biomolecules.Frontiers in plant science · 2025Review
- Plants Utilize a Protection/Deprotection Strategy in Limonoid Biosynthesis: A "Missing Link" Carboxylesterase Boosts Yields and Provides Insights into Furan Formation.Journal of the American Chemical Society · 2024Article
- Nepeta cataria L. (catnip) can serve as a chassis for the engineering of secondary metabolic pathways.Biotechnology letters · 2024Article
- Characterization of a vacuolar importer of secologanin in Catharanthus roseus.Communications biology · 2024Article
- Cutting-edge plant natural product pathway elucidation.Current opinion in biotechnology · 2024Review
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
Monoterpene indole alkaloids (MIAs) are a diverse class of plant natural products that include a number of medicinally important compounds. We set out to reconstitute the pathway for strictosidine, a key intermediate of all MIAs, from central metabolism in Nicotiana benthamiana. A disadvantage of this host is that its rich background metabolism results in the derivatization of some heterologously produced molecules. Here we use transcriptomic analysis to identify glycosyltransferases that are upregulated in response to biosynthetic intermediates and produce plant lines with targeted mutations in the genes encoding them. Expression of the early MIA pathway in these lines produces a more favorable product profile. Strictosidine biosynthesis was successfully reconstituted, with the best yields obtained by the co-expression of 14 enzymes, of which a major latex protein-like enzyme (MLPL) from Nepeta (catmint) is critical for improving flux through the iridoid pathway. The removal of endogenous glycosyltransferases does not impact the yields of strictosidine, highlighting that the metabolic flux of the pathway enzymes to a stable biosynthetic intermediate minimizes the need to engineer the endogenous metabolism of the host. The production of strictosidine in planta expands the range of MIA products amenable to biological synthesis.
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