ArticleBiophysical journal2022
Spectroscopic and QM/MM studies of the Cu(I) binding site of the plant ethylene receptor ETR1.
Article in Biophysical journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Integrative approaches for the structure-based functional understanding of the ethylene response in plants.The Plant journal : for cell and molecular biology · 2026Review
- Structural dynamics of the plant hormone receptor ETR1 in a native-like membrane environment.FEBS letters · 2025Article
- Modulation of plant growth and development through altered ethylene binding affinity of the ethylene receptor ETR1.BMC plant biology · 2025Article
- Biological formation of ethylene.RSC chemical biology · 2023Review
- Basis for high-affinity ethylene binding by the ethylene receptor ETR1 of Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Herein, we present, to our knowledge, the first spectroscopic characterization of the Cu(I) active site of the plant ethylene receptor ETR1. The x-ray absorption (XAS) and extended x-ray absorption fine structure (EXAFS) spectroscopies presented here establish that ETR1 has a low-coordinate Cu(I) site. The EXAFS resolves a mixed first coordination sphere of N/O and S scatterers at distances consistent with potential histidine and cysteine residues. This finding agrees with the coordination of residues C65 and H69 to the Cu(I) site, which are critical for ethylene activity and well conserved. Furthermore, the Cu K-edge XAS and EXAFS of ETR1 exhibit spectroscopic changes upon addition of ethylene that are attributed to modifications in the Cu(I) coordination environment, suggestive of ethylene binding. Results from umbrella sampling simulations of the proposed ethylene binding helix of ETR1 at a mixed quantum mechanics/molecular mechanics level agree with the EXAFS fit distance changes upon ethylene binding, particularly in the increase of the distance between H69 and Cu(I), and yield binding energetics comparable with experimental dissociation constants. The observed changes in the copper coordination environment might be the triggering signal for the transmission of the ethylene response.
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