Evidence map›Paper›PMID 36085283›Full record

ArticleCell discovery2022

Structural insights into human CCAN complex assembled onto DNA.

Tian Tian, Lili Chen, Zhen Dou, Zhisen Yang, Xinjiao Gao, Xiao Yuan, Chengliang Wang, Ran Liu, Zuojun Shen, Ping Gui and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell discovery, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
4.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 48 citations in OpenAlex.

  1. Structure and function of the centromere and inner kinetochore.Nature reviews. Molecular cell biology · 2026
    Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Structure and dynamics of 2x(CENP-A/H4)Life science alliance · 2026
    Article
  7. Article
  8. The dynamic centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Non-nucleosomal (CENP-A/H4)bioRxiv : the preprint server for biology · 2025
    Article
  18. Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 2 countries.

Tian Tian *MOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Lili Chen *MOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Zhen Dou *MOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Zhisen Yang *MOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Xinjiao GaoMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China. gaox@ustc.edu.cn.ORCID http://orcid.org/0000-0002-9567-5937
Xiao YuanMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Chengliang WangMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Ran LiuMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Zuojun ShenMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.ORCID http://orcid.org/0000-0001-7052-5652
Ping GuiMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Maikun TengMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Xianlei MengMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Donald L HillComprehensive Cancer Center, University of Alabama, Birmingham, AL, USA.
Lin LiCAS Center for Excellence in Molecular and Cell Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-3821-7147
Xuan ZhangMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.
Xing LiuMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China.ORCID http://orcid.org/0000-0002-7480-5085
Linfeng SunMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China. sunlf17@ustc.edu.cn.ORCID http://orcid.org/0000-0001-9367-2444
Jianye ZangMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China. zangjy@ustc.edu.cn.ORCID http://orcid.org/0000-0003-2648-9783
Xuebiao YaoMOE Key Laboratory for Cellular Dynamics, the First Affiliated Hospital, CAS Center for Excellence in Biomacromolecules, and School of Life Sciences, University of Science and Technology of China, Hefei, China. yaoxb@ustc.edu.cn.
University of Science and Technology of China · CNUniversity of Alabama at Birmingham · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In mitosis, accurate chromosome segregation depends on kinetochores that connect centromeric chromatin to spindle microtubules. The centromeres of budding yeast, which are relatively simple, are connected to individual microtubules via a kinetochore constitutive centromere associated network (CCAN). However, the complex centromeres of human chromosomes comprise millions of DNA base pairs and attach to multiple microtubules. Here, by use of cryo-electron microscopy and functional analyses, we reveal the molecular basis of how human CCAN interacts with duplex DNA and facilitates accurate chromosome segregation. The overall structure relates to the cooperative interactions and interdependency of the constituent sub-complexes of the CCAN. The duplex DNA is topologically entrapped by human CCAN. Further, CENP-N does not bind to the RG-loop of CENP-A but to DNA in the CCAN complex. The DNA binding activity is essential for CENP-LN localization to centromere and chromosome segregation during mitosis. Thus, these analyses provide new insights into mechanisms of action underlying kinetochore assembly and function in mitosis.

Identifiers

PMID36085283
PMCPMC9463443
OpenAlexW4295067203

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.