ArticleEpigenetics & chromatin2022
Sperm DNA methylation alterations from cannabis extract exposure are evident in offspring.
Article in Epigenetics & chromatin, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Progeny effects of rotenone exposure depend on parental toxicity.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- Environmental and Genetic Perturbations of the Sperm Epigenome.Advances in experimental medicine and biology · 2026Review
- Review
- Article
- The impact of cannabinoids on reproductive function.Reproduction (Cambridge, England) · 2025Review
- Prenatal cannabis exposure is associated with alterations in offspring DNA methylation at genes involved in neurodevelopment, across the life course.Molecular psychiatry · 2025Article
- Extended abstinence from morphine alters sperm smRNA expression and prevents transmission of intergenerational phenotypes.Environmental epigenetics · 2025Article
- Tackling somatic DNA contamination in sperm epigenetic studies.Frontiers in reproductive health · 2025Article
- Key insights into cannabis-cancer pathobiology and genotoxicity.Addiction biology · 2024Article
- Influence of substance use on male reproductive health and offspring outcomes.Nature reviews. Urology · 2024Review
- Persisting neurobehavioral consequences of daily or intermittent paternal cannabis administration in F1 and F2 Rats.Neurotoxicology · 2024Article
- A new approach to study stochastic epigenetic mutations in sperm methylome of Vietnam war veterans directly exposed to Agent Orange.Environmental epigenetics · 2024Article
- THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modifications.PloS one · 2024Article
- Paternal impact on the developmental programming of sexual dimorphism.Frontiers in cell and developmental biology · 2024Review
- Cannabis- and Substance-Related Carcinogenesis in Europe: A Lagged Causal Inferential Panel Regression Study.Journal of xenobiotics · 2023Article
- Early life adversity: Epigenetic regulation underlying drug addiction susceptibility.Molecular and cellular neurosciences · 2023Review
- Cannabis and Paternal Epigenetic Inheritance.International journal of environmental research and public health · 2023Review
- An epigenetic synopsis of parental substance use.Epigenomics · 2023Review
- Emerging Roles of Endocannabinoids as Key Lipid Mediators for a Successful Pregnancy.International journal of molecular sciences · 2023Review
- Sperm capacitation and transcripts levels are altered by in vitro THC exposure.BMC molecular and cell biology · 2023Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCannabis legalization is expanding and men are the predominant users. We have limited knowledge about how cannabis impacts sperm and whether the effects are heritable.
resultsWhole genome bisulfite sequencing (WGBS) data were generated for sperm of rats exposed to: (1) cannabis extract (CE) for 28 days, then 56 days of vehicle only (~ one spermatogenic cycle); (2) vehicle for 56 days, then 28 days of CE; or (3) vehicle only. Males were then mated with drug-naïve females to produce F1 offspring from which heart, brain, and sperm tissues underwent analyses. There were 3321 nominally significant differentially methylated CpGs in F0 sperm identified via WGBS with select methylation changes validated via bisulfite pyrosequencing. Significant methylation changes validated in F0 sperm of the exposed males at the gene 2-Phosphoxylose Phosphatase 1 (Pxylp1) were also detectable in their F1 sperm but not in controls. Changes validated in exposed F0 sperm at Metastasis Suppressor 1-Like Protein (Mtss1l) were also present in F1 hippocampal and nucleus accumbens (NAc) of the exposed group compared to controls. For Mtss1l, a significant sex-specific relationship between DNA methylation and gene expression was demonstrated in the F1 NAc. Phenotypically, rats born to CSE-exposed fathers exhibited significant cardiomegaly relative to those born to control fathers.
conclusionsThis is the first characterization of the effect of cannabis exposure on the entirety of the rat sperm methylome. We identified CE-associated methylation changes across the sperm methylome, some of which persisted despite a "washout" period. Select methylation changes validated via bisulfite pyrosequencing, and genes associated with methylation changes were involved in early developmental processes. Preconception CE exposure is associated with detectable changes in offspring DNA methylation that are functionally related to changes in gene expression and cardiomegaly. These results support that paternal preconception exposure to cannabis can influence offspring outcomes.
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