Evidence map›Paper›PMID 36085198›Full record

ArticleArchives of microbiology2022

Long-term survive of Aliarcobacter butzleri in two models symbiotic interaction with Acanthamoeba castellanii.

Gustavo A Medina, Sandra N Flores-Martin, Wellison A Pereira, Elías G Figueroa, Neftalí H Guzmán, Pablo J Letelier, Marcela R Andaur, Pilar I Leyán, Rodrigo E Boguen, Alfonso H Hernández and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Gustavo A MedinaPrecision Health Research Laboratory, Departamento de Procesos Diagnósticos y Evaluación, Facultad de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile. *gmedina@uct.cl.ORCID http://orcid.org/0000-0003-3889-1745
Sandra N Flores-MartinInstitute of Clinical Microbiology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile.ORCID http://orcid.org/0000-0003-0490-0578
Wellison A PereiraLaboratory of Microbial Biomolecules, Faculty of Pharmaceutical Sciences, University of São Paulo, Rua Do Lago, 250, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.ORCID http://orcid.org/0000-0002-7002-0811
Elías G FigueroaNucleus of Research in Food Production, Faculty of Natural Resources, Universidad Católica de Temuco, Temuco, Chile.ORCID http://orcid.org/0000-0002-3979-7876
Neftalí H GuzmánPrecision Health Research Laboratory, Departamento de Procesos Diagnósticos y Evaluación, Facultad de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile.ORCID http://orcid.org/0000-0003-4872-9821
Pablo J LetelierPrecision Health Research Laboratory, Departamento de Procesos Diagnósticos y Evaluación, Facultad de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile.ORCID http://orcid.org/0000-0001-9803-9752
Marcela R AndaurPrecision Health Research Laboratory, Departamento de Procesos Diagnósticos y Evaluación, Facultad de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile.ORCID http://orcid.org/0000-0002-8538-2225
Pilar I LeyánPrecision Health Research Laboratory, Departamento de Procesos Diagnósticos y Evaluación, Facultad de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile.ORCID http://orcid.org/0000-0002-0995-3663
Rodrigo E BoguenPrecision Health Research Laboratory, Departamento de Procesos Diagnósticos y Evaluación, Facultad de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile.ORCID http://orcid.org/0000-0002-0325-743X
Alfonso H HernándezPrecision Health Research Laboratory, Departamento de Procesos Diagnósticos y Evaluación, Facultad de Ciencias de la Salud, Universidad Católica de Temuco, Temuco, Chile.ORCID http://orcid.org/0000-0001-7292-5385
Heriberto FernándezInstitute of Clinical Microbiology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, Chile.ORCID http://orcid.org/0000-0002-0009-7258

Funding

Fondo Concurso Interno Línea Profondecyt, Vicerrectoría de Investigación y Postgrado, Universidad Católica De Temuco VIPUCT, #2016PF-GM-03Fondo de Equipamiento Vicerrectoría de Investigación y Posgrado Universidad Católica de Temuco FEQUIP2019-CS-05Fondo Nacional de Desarrollo Científico y Tecnológico 1110202
6 · The paper itself

Abstract

Aliarcobacter butzleri (formerly known as Arcobacter butzleri) is an emerging food-borne zoonotic pathogen that establishes in vitro endosymbiotic relationships with Acanthamoeba castellanii, a free-living amoeba. Previously, we described that this bacterium acts as an endocytobiont of A. castellanii, surviving for at least 10 days in absence of bacterial replication. Thus, the aim of this study was to evaluate the ability of A. butzleri to survive as a long-term endosymbiont of A. castellanii for 30 days in two models of symbiotic interaction with A. castellanii: (i) endosymbiotic culture followed by gentamicin protection assay and (ii) transwell co-culture assay. The results allow us to conclude that A. butzleri is capable of surviving as an endosymbiont of A. castellanii for at least 30 days, without multiplying, under controlled laboratory conditions. In addition, in the absence of nutrients and as both microorganisms remain in the same culture, separated by semi-permeable membranes, A. castellanii does not promote the survival of A. butzleri, nor does it multiply. Our findings suggest that the greater survival capacity of A. butzleri is associated with their endosymbiont status inside A. castellanii, pointing out the complexity of this type of symbiotic relationship.

Indexed as

Acanthamoeba castellaniiArcobacterSymbiosisAcanthamoebaAliarcobacterEndosymbiosis

Identifiers

PMID36085198

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.