Evidence map›Paper›PMID 36084727›Full record

ArticleExperimental eye research2022

Revealing the presence of tear extracellular vesicles in Keratoconus.

Brenna S Hefley, Clayton Deighan, Brenda Vasini, Asher Khan, Jesper Hjortdal, Kamran M Riaz, Yutao Liu, Dimitrios Karamichos

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
5.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
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  10. Characterizing Extracellular Vesicles from Biological Fluids.Journal of visualized experiments : JoVE · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Brenna S HefleyNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA; Department of Pharmaceutical Sciences, College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA.
Clayton DeighanNanoView Biosciences, Boston, MA, USA.
Brenda VasiniNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA; Department of Pharmaceutical Sciences, College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA.
Asher KhanDepartment of Ophthalmology, Dean McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Jesper HjortdalDepartment of Ophthalmology, Aarhus University Hospital, Palle Juul-Jensens Boulevard 167, 8200, Aarhus N, Denmark.
Kamran M RiazDepartment of Ophthalmology, Dean McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Yutao LiuDepartment of Cellular Biology and Anatomy, Augusta University, Augusta, GA, USA; James & Jean Culver Vision Discovery Institute, Augusta University, Augusta, GA, USA; Center for Biotechnology and Genomic Medicine, Augusta University, Augusta, GA, USA.
Dimitrios KaramichosNorth Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA; Department of Pharmaceutical Sciences, College of Pharmacy, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA; Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, TX, 76107, USA. Electronic address: dimitrios.karamichos@unthsc.edu.
University of North Texas · USDean McGee Eye Institute · USAarhus University Hospital · DKAugusta University · US

Funding

Module 3: Gene Expression/ProteomicsP30EY031631 · NEI · AUGUSTA UNIVERSITY · PI Xingjun Fan · 2020 to 2026
$3.6M
Gene Discovery in Familial KeratoconusR01EY023242 · NEI · DUKE UNIVERSITY · PI LIU, YUTAO · 2013 to 2023
$3.4M
Utility of PIP as a Novel Keratoconus Biomarker | SupplementR01EY030028 · NEI · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · PI KARAMICHOS, DIMITRIOS · 2019 to 2023
$1.7M
Long Noncoding RNA lncLOXL1 and Exfoliation Syndrome.R21EY028671 · NEI · AUGUSTA UNIVERSITY · PI LIU, YUTAO · 2018 to 2019
$467k
NEI NIH HHS P30 EY031631NEI NIH HHS R01 EY023242NEI NIH HHS R01 EY030028NEI NIH HHS R21 EY028671
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are lipid-bound vesicles that originate from the endosomal system or budded off from the plasma membrane. EVs are involved in cell-cell communication via transporting DNA, RNA, and proteins from one cell to another. Tear EVs (tEVs) have been reported in dry eye, Sjӧgren's Syndrome, and primary open-angle glaucoma. In this study, we sought to investigate the presence of tEVs in relation to keratoconus (KC). Tears were passively collected from the lateral meniscus from 10 healthy (5 males and 5 females) and 9 KC (4 males and 5 females) subjects. Tear samples were processed and analyzed using the ExoView™ R100. Statistical analysis was performed using a Mann-Whitney U non-parametric Student's t-test. All tEVs, in both Healthy and KC subjects, showed a CD9

Indexed as

Extracellular VesiclesGlaucoma, Open-AngleKeratoconusFemaleHumansLipidsMaleRNATearsLipidsRNACorneaExtracellular vesiclesKeratoconusPhenotypeTearTetraspanin

Identifiers

PMID36084727
PMCPMC10159047
OpenAlexW4294770887

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.