ArticleVeterinary medicine and science2022
Co-culturing or conditioned medium of Sertoli cells: Which one supports in vitro maturation of bovine oocytes and developmental competency of resulting embryos?
Article in Veterinary medicine and science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 3 citations in OpenAlex.
- Advances in bovine oocyte in vitro maturation: mechanisms and strategies.Veterinary research communications · 2026Review
- Improving Bovine Embryo Development and Quality Using Bovine Oviductal Epithelial Cell-Derived Conditioned Medium (bOEC-CM).Veterinary medicine and science · 2025Article
- Co-culturing or conditioned medium of Sertoli cells: Which one supports in vitro maturation of bovine oocytes and developmental competency of resulting embryos?Veterinary medicine and science · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundSertoli cells (SCs) as supportive cells in the seminiferous tubule play an essential role in the nutrition and development of adjacent cells by secreting several beneficial growth factors, stimulators and cytokines which can be conceived to improve the developmental competency of oocyte or embryo in the co-culture system.
objectivesThis study aimed to improve the maturation of bovine oocytes and consequently the development of resulting embryos in co-culture with SCs and their conditioned medium (CM).
methodsThe retrieved cumulus-oocyte complexes (COCs) from the abattoir-derived ovaries were matured in maturation medium alone (control group), in co-culture with ovine SCs (co-culture group), and in presence of 10% CM prepared in 33°C and 39°C (CM33 and CM39 groups). The nuclear maturation competency and subsequent embryo development rate of cultured COCs in all groups were evaluated.
resultsThe results of this study showed that SCs and CM increased meiosis resumption from GV to the MII compared to the control group, significantly (p < 0.05). Besides, the degenerated oocytes in the co-culture group were significantly higher than those in the control, CM33 and CM39 groups (p < 0.05), and the lowest cleavage rate belonged to the co-culture group (p < 0.05). The blastocyst rate was also lower in the co-culture group than other groups and there was a significant difference between the control and two CM groups (p < 0.05).
conclusionsThe Sertoli cells can be suitable for co-culturing with oocytes during IVM but detrimental for subsequent embryo development. In turn, Sertoli cell-derived conditioned medium (SC-CM) can provide sufficient bioactive materials for COCs to enhancing oocyte competence and embryo development.
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