Evidence map›Paper›PMID 36084194›Full record

ArticleJournal of the American Chemical Society2022

Macromolecular Crowding as an Intracellular Stimulus for Responsive Nanomaterials.

Daniel A Estabrook, John O Chapman, Shuo-Ting Yen, Helen H Lin, Ethan T Ng, Linglan Zhu, Heidi L van de Wouw, Otger Campàs, Ellen M Sletten

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Non-Ionic Fluorosurfactants for Droplet-Based in vivo Applications.Angewandte Chemie (International ed. in English) · 2024
    Article
  5. ACS omega · 2024
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Daniel A EstabrookDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0001-7237-6291
John O ChapmanDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0001-5252-0962
Shuo-Ting YenDepartment of Mechanical Engineering, University of California, Santa Barbara, California 93106, United States.ORCID 0000-0003-4277-1965
Helen H LinDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
Ethan T NgDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
Linglan ZhuDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
Heidi L van de WouwDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0002-2115-6325
Otger CampàsDepartment of Mechanical Engineering, University of California, Santa Barbara, California 93106, United States.
Ellen M SlettenDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.ORCID 0000-0002-0049-7278

Funding

Biotechnology Training in Biomedical Sciences and EngineeringT32GM067555 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GU, ZHEN · 2005 to 2019
$2.8M
Robust microdroplet-based mechanical probes for wide-ranging mechanobiology applicationsR01GM135380 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CAMPAS, OTGER, SLETTEN, ELLEN MAY · 2019 to 2022
$1.8M
NIGMS NIH HHS R01 GM135380NIGMS NIH HHS T32 GM067555
6 · The paper itself

Abstract

Stimuli-responsive materials are exploited in biological, materials, and sensing applications. We introduce a new endogenous stimulus, biomacromolecule crowding, which we achieve by leveraging changes in thermoresponsive properties of polymers upon high concentrations of crowding agents. We prepare poly(2-oxazoline) amphiphiles that exhibit lower critical solution temperatures (LCST) in serum above physiological temperature. These amphiphiles stabilize oil-in-water nanoemulsions at temperatures below the LCST but are ineffective surfactants above the LCST, resulting in emulsion fusion. We find that the transformations observed upon heating nanoemulsions above their surfactant's LCST can instead be induced at physiological temperatures through the addition of polymers and protein, rendering thermoresponsive materials "crowding responsive." We demonstrate that the cytosol is a stimulus for nanoemulsions, with droplet fusion occurring upon injection into cells of living zebrafish embryos. This report sets the stage for classes of thermoresponsive materials to respond to macromolecule concentration rather than temperature changes.

Indexed as

NanostructuresStimuli Responsive PolymersAnimalsEmulsionsPolymersSurface-Active AgentsTemperatureWaterZebrafishEmulsionsPolymersStimuli Responsive PolymersSurface-Active AgentsWater

Identifiers

PMID36084194
PMCPMC9583728

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.