Evidence map›Paper›PMID 36082977›Full record

ArticleChemistry (Weinheim an der Bergstrasse, Germany)2022

Phomoxanthone A Targets ATP Synthase.

Rameez Ali, Sangram S Parelkar, Paul R Thompson, Susan Mitroka-Batsford, Siddartha Yerramilli, Suzanne F Scarlata, Katelyn S Mistretta, Jeannine M Coburn, Anita E Mattson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Rameez AliDepartment of Chemistry and Biochemistry, Worcester Polytechnic Institute, 60 Prescott St., Worcester, MA 01609, USA.
Sangram S ParelkarDepartment of Chemical Biology, University of Massachusetts Medical School, 364 Plantation St., Wocester, MA 01605, USA.
Paul R ThompsonDepartment of Chemical Biology, University of Massachusetts Medical School, 364 Plantation St., Wocester, MA 01605, USA.
Susan Mitroka-BatsfordDepartment of Chemistry and Biochemistry, Worcester State University, 486 Chandler St., Worcester, MA 10602, USA.
Siddartha YerramilliDepartment of Chemistry and Biochemistry, Worcester Polytechnic Institute, 60 Prescott St., Worcester, MA 01609, USA.
Suzanne F ScarlataDepartment of Chemistry and Biochemistry, Worcester Polytechnic Institute, 60 Prescott St., Worcester, MA 01609, USA.
Katelyn S MistrettaDepartment of Biomedical Engineering, Worcester Polytechnic Institute, 60 Prescott St., Worcester, MA 01609, USA.
Jeannine M CoburnDepartment of Biomedical Engineering, Worcester Polytechnic Institute, 60 Prescott St., Worcester, MA 01609, USA.
Anita E MattsonDepartment of Chemistry and Biochemistry, Worcester Polytechnic Institute, 60 Prescott St., Worcester, MA 01609, USA.ORCID http://orcid.org/0000-0003-4662-3468
Worcester Polytechnic Institute · USUniversity of Massachusetts Chan Medical School · USWorcester State University · US

Funding

Chemical probes to decipher PAD biologyR35GM118112 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Paul R Thompson · 2016 to 2026
$9.5M
Silanediol Enabled Drug DiscoveryR35GM124804 · NIGMS · WORCESTER POLYTECHNIC INSTITUTE · PI MATTSON, ANITA E · 2017 to 2021
$2.0M
NIGMS NIH HHS R35 GM118112NIGMS NIH HHS R35 GM124804NIH HHS R35GM124804
6 · The paper itself

Abstract

Phomoxanthone A is a naturally occurring molecule and a powerful anti-cancer agent, although its mechanism of action is unknown. To facilitate the determination of its biological target(s), we used affinity-based labelling using a phomoxanthone A probe. Labelled proteins were pulled down, subjected to chemoproteomics analysis using LC-MS/MS and ATP synthase was identified as a likely target. Mitochondrial ATP synthase was validated in cultured cells lysates and in live intact cells. Our studies show sixty percent inhibition of ATP synthase by 260 μM phomoxanthone A.

Indexed as

Mitochondrial Proton-Translocating ATPasesTandem Mass SpectrometryAdenosine TriphosphateAffinity LabelsChromatography, LiquidXanthonesAdenosine TriphosphateAffinity LabelsMitochondrial Proton-Translocating ATPasesphomoxanthone AXanthonesanticancerbiconjugationmicroscopynatural productphotoaffinity labelling

Identifiers

PMID36082977
PMCPMC9942271
OpenAlexW4295048427

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.