ArticleFrontiers in cell and developmental biology2022
Capacitation promotes a shift in energy metabolism in murine sperm.
Article in Frontiers in cell and developmental biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Exploring Human Sperm Metabolism and Male Infertility: A Systematic Review of Genomics, Proteomics, Metabolomics, and Imaging Techniques.International journal of molecular sciences · 2025Pooled it
- Single-cell metabolic profiling of stallion spermatozoa by flow cytometry using NADH and FAD autofluorescence†.Biology of reproduction · 2026Article
- Sperm mitochondrial sheath formation - how and why?Nature reviews. Urology · 2026Review
- Suppression of mitochondrial energy production by a photosynthetic bacterial cupredoxin peptide inhibits tumor growth.Signal transduction and targeted therapy · 2026Article
- Targeted metabolomics reveals the impact of glucose and pyruvate on energy metabolism and storage potential of stallion spermatozoa.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Metabolic pathways fueling human spermatozoa capacitation and hyperactivation: a roadmap for spermatozoa bioenergetics.Frontiers in endocrinology · 2026Review
- Increasing the fertility of Sapudi sheep semen at 5°C storage temperature with the addition of oxytocin in a diluent of skim milk, egg yolk, and citrate.Open veterinary journal · 2025Article
- A novel mechanism of sperm midpiece epididymal maturation and the role of CCDC112 in sperm midpiece formation and establishing an optimal flagella waveform.Cell communication and signaling : CCS · 2025Article
- Equine in vitro fertilization with frozen-thawed semen is associated with shortened pre-incubation time and modified capacitation-related changes.Biology of reproduction · 2025Article
- Capacitation of ram spermatozoa promotes changes in energy metabolism and aquaporin 3 and is affected by individual testosterone variations.Andrology · 2025Article
- Bioenergetics of Rodent Spermatozoa.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Integrating proteomics and metabolomics to evaluate impact of semen collection techniques on the quality and cryotolerance of goat semen.Scientific reports · 2024Article
- Reproductive Tract Mucus May Influence the Sex of Offspring in Cattle: Study in Cows That Have Repeatedly Calved Single-Sex Offspring.Veterinary sciences · 2024Article
- Adenylate kinase 9 is essential for sperm function and male fertility in mammals.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model.Communications biology · 2023Article
- Alkaline Dilution Alters Sperm Motility in Dairy Goat by Affecting sAC/cAMP/PKA Pathway Activity.International journal of molecular sciences · 2023Article
- Capacitation induces changes in metabolic pathways supporting motility of epididymal and ejaculated sperm.Frontiers in cell and developmental biology · 2023Article
- Effect of High Viscosity on Energy Metabolism and Kinematics of Spermatozoa from Three Mouse Species Incubated under Capacitating Conditions.International journal of molecular sciences · 2022Article
- The sperm mitochondria: clues and challenges.Animal reproduction · 2022Article
- Molecular mechanisms of mammalian sperm capacitation, and its regulation by sodium-dependent secondary active transporters.Reproductive medicine and biologyReview
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4 authors.
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Abstract
In mammals, sperm acquire fertilization ability after a series of physiological and biochemical changes, collectively known as capacitation, that occur inside the female reproductive tract. In addition to other requirements, sperm bioenergetic metabolism has been identified as a fundamental component in the acquisition of capacitation. Mammalian sperm produce ATP through two main metabolic processes, oxidative phosphorylation (OXPHOS) and aerobic glycolysis that are localized to two different flagellar compartments, the midpiece, and the principal piece, respectively. In mouse sperm, the occurrence of many events associated with capacitation relies on the activity of these two energy-producing pathways, leading to the hypothesis that some of these events may impose changes in sperm energetic demands. In the present study, we used extracellular flux analysis to evaluate changes in glycolytic and respiratory parameters of murine sperm that occur as a consequence of capacitation. Furthermore, we examined whether these variations affect sperm ATP sustainability. Our results show that capacitation promotes a shift in the usage ratio of the two main metabolic pathways, from oxidative to glycolytic. However, this metabolic rewiring does not seem to affect the rate at which the sperm consume ATP. We conclude that the probable function of the metabolic switch is to increase the ATP supply in the distal flagellar regions, thus sustaining the energetic demands that arise from capacitation.
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