Evidence map›Paper›PMID 36080398›Full record

ArticleMolecules (Basel, Switzerland)2022

Biopreservation of Chocolate Mousse with Lactobacillus helveticus 2/20: Microbial Challenge Test

Bogdan Goranov, Desislava Teneva, Rositsa Denkova-Kostova, Vesela Shopska, Nadia Oulahal, Zapryana Denkova, Georgi Kostov, Pascal Degraeve, Rafael Pagan

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Evaluation ofMicroorganisms · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Bogdan GoranovDepartment "Microbiology", University of Food Technologies-Plovdiv, 26 Maritza Boulevard, 4002 Plovdiv, Bulgaria.
Desislava TenevaLaboratory of Biologically Active Substances, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 139 Ruski Boulevard, 4000 Plovdiv, Bulgaria.ORCID 0000-0003-3109-2238
Rositsa Denkova-KostovaDepartment "Technology Biochemistry and Molecular Biology", University of Food Technologies-Plovdiv, 26 Maritza Boulevard, 4002 Plovdiv, Bulgaria.
Vesela ShopskaDepartment "Technology of Wine and Beer", University of Food Technologies-Plovdiv, 26 Maritza Boulevard, 4002 Plovdiv, Bulgaria.ORCID 0000-0002-4492-5094
Nadia OulahalBioingénierie et Dynamique Microbienne aux Interfaces Alimentaires Research Unit, IUT Lyon 1, Technopole Alimentec, Université Claude Bernard Lyon 1, ISARA Lyon, 155 rue Henri de Boissieu, F-01000 Bourg en Bresse, France.ORCID 0000-0002-6965-7487
Zapryana DenkovaDepartment "Microbiology", University of Food Technologies-Plovdiv, 26 Maritza Boulevard, 4002 Plovdiv, Bulgaria.
Georgi KostovDepartment "Technology of Wine and Beer", University of Food Technologies-Plovdiv, 26 Maritza Boulevard, 4002 Plovdiv, Bulgaria.ORCID 0000-0003-4661-0421
Pascal DegraeveBioingénierie et Dynamique Microbienne aux Interfaces Alimentaires Research Unit, IUT Lyon 1, Technopole Alimentec, Université Claude Bernard Lyon 1, ISARA Lyon, 155 rue Henri de Boissieu, F-01000 Bourg en Bresse, France.ORCID 0000-0001-5357-3433
Rafael PaganDepartamento de Producción Animal y Ciencia de los Alimentos, Facultad de Veterinaria, Instituto Agroalimentario de Aragón-IA2, Universidad de Zaragoza-CITA, Calle Miguel Servet, 177, 50013 Zaragoza, Spain.ORCID 0000-0002-0238-6328
University of Food Technologies · BGUniversité Claude Bernard Lyon 1 · FRBulgarian Academy of Sciences · BGUniversidad de Zaragoza · ES

Funding

Strengthening the research excellence and innovation capacity of University of Food Technologies - Plovdiv, through the sustainable development of tailor-made food systems with programmable properties", part of the European Scientific Networks National Pr Д01-288/07.10.2020the Ministry of Education and Science of the Republic of Bulgaria under the National Research Programme "Healthy Foods for a Strong Bio-Economy and Quality of Life" DCM № 577/17.08.2018
6 · The paper itself

Abstract

Probiotic bacteria are used for food biopreservation because their metabolic products might contribute to ensuring food microbiological safety and/or increase its shelf life without the addition of chemical preservatives. Moreover, biopreserved foods are excellent vehicles for the delivery of probiotic bacteria. The aim of the study was to investigate the potential of chocolate mousse food matrix for the delivery of the probiotic strain Lactobacillus helveticus 2/20 (Lb. helveticus 2/20) and to investigate its capacity to inhibit the growth of two foodborne pathogenic bacteria (Staphylococcus aureus and Escherichia coli). Therefore, the populations of free or encapsulated in calcium alginate Lb. helveticus 2/20 cells and/or of each pathogen (used to voluntarily contaminate each sample) were monitored both in complex nutrient medium (MRS broth) and in chocolate mousse under refrigeration conditions and at room temperature. Lb. helveticus 2/20 alone in free or encapsulated state effectively inhibited the growth of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 in chocolate mousse when stored at 20 ± 2 °C. Practically no viable unwanted bacteria were identified on the 7th day from the beginning of the process. High viable Lb. helveticus 2/20 cell populations were maintained during storage under refrigerated conditions (4 ± 2 °C) and at room temperature. Chocolate mousse is thus a promising food matrix to deliver probiotic Lb. helveticus 2/20 cells, which could also protect it from contamination by unwanted bacteria.

Indexed as

ChocolateLactobacillus helveticusProbioticsEscherichia colibiopreservationchocolate moussemicrobial challenge testprobiotics

Identifiers

PMID36080398
PMCPMC9457945
OpenAlexW4294162940

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.