Evidence map›Paper›PMID 36078076›Full record

ArticleCells2022

Cryopreservation and Cryobanking of Cells from 100 Coral Species.

En-Chun Toh, Kuan-Lin Liu, Sujune Tsai, Chiahsin Lin

Open access · goldAbstract read
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

En-Chun TohInstitute of Marine Biology, National Dong Hwa University, Pingtung 944401, Taiwan.
Kuan-Lin LiuInstitute of Marine Biology, National Dong Hwa University, Pingtung 944401, Taiwan.
Sujune TsaiDepartment of Post Modern Agriculture, Mingdao University, Peetow, Changhua 52345, Taiwan.
Chiahsin LinInstitute of Marine Biology, National Dong Hwa University, Pingtung 944401, Taiwan.
National Dong Hwa University · TWMingDao University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

When coral species become extinct, their genetic resources cannot be recovered. Coral cryobanks can be employed to preserve coral samples and thereby maintain the availability of the samples and increase their potential to be restocked. In this study, we developed a procedure to determine coral species-specific requirements for cryobank freezing through determining suitable cryoprotective agents (CPAs), CPA concentrations, equilibration times, holding durations, viability rates, and cell amounts for banked coral cells, and we established the first ever coral cell cryobank. Coral cells, including supporting and gland cells, epidermal nematocysts, Symbiodiniaceae and symbiotic endoderm cells (SEC) were found from the extracted protocol. Approximately half of the corals from the experimental corals consisted of spindle and cluster cells. Gastrodermal nematocysts were the least common. The overall concentration of Symbiodiniaceae in the coral cells was 8.6%. Freezing using DMSO as a CPA was suitable for approximately half of the corals, and for the other half of species, successful cell cryopreservation was achieved using MeOH and EG. EG and DMSO had similar suitabilities for

Indexed as

AnthozoaAnimalsCryopreservationCryoprotective AgentsDimethyl SulfoxideFreezingCryoprotective AgentsDimethyl SulfoxidecellcoralcryobankcryopreservationcryoprotectantSymbiodiniaceae

Identifiers

PMID36078076
PMCPMC9454506
OpenAlexW4293664171

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.