Evidence map›Paper›PMID 36076861›Full record

ArticleFoods (Basel, Switzerland)2022

PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens.

Mengtao Chen, Xinyue Lan, Longjiao Zhu, Ping Ru, Wentao Xu, Haiyan Liu

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Flow cytometry study ofFrontiers in microbiology · 2025
    Article
  8. Advancements in Detection Methods forPathogens (Basel, Switzerland) · 2024
    Review
  9. Article
  10. Article
  11. Review
  12. The Rapid Detection ofFoods (Basel, Switzerland) · 2023
    Article
  13. Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023
    Review
  14. Review
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mengtao ChenResearch Center for Sports Nutrition and Eudainomics, Institute for Sports Training Science, Tianjin University of Sport, Tianjin 301617, China.
Xinyue LanKey Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health (Institute of Nutrition and Health), China Agricultural University, Beijing 100193, China.
Longjiao ZhuKey Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health (Institute of Nutrition and Health), China Agricultural University, Beijing 100193, China.
Ping RuResearch Center for Sports Nutrition and Eudainomics, Institute for Sports Training Science, Tianjin University of Sport, Tianjin 301617, China.
Wentao XuKey Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health (Institute of Nutrition and Health), China Agricultural University, Beijing 100193, China.ORCID 0000-0002-2302-8490
Haiyan LiuResearch Center for Sports Nutrition and Eudainomics, Institute for Sports Training Science, Tianjin University of Sport, Tianjin 301617, China.

Funding

the Hebei Province key research and development program 21372801Dthe National Key Research and Development Program No. 2018YFC1603704the National Natural Science Foundation of China No. 31671922the National Natural Science Foundation of China No. 31871875the Young Elite Scientist Sponsorship Program by Bast NO.BYESS2022133
6 · The paper itself

Abstract

Living foodborne pathogens pose a serious threat to public and population health. To ensure food safety, it is necessary to complete the detection of viable bacteria in a short time (several hours to 1 day). However, the traditional methods by bacterial culture, as the gold standard, are cumbersome and time-consuming. To break through the resultant research bottleneck, PCR mediated nucleic acid molecular recognition technologies, including RNA-based reverse transcriptase PCR (RT-PCR) and DNA-based viability PCR (vPCR) have been developed in recent years. They not only sensitively amplify detection signals and quickly report detection results, but also distinguish viable and dead bacteria. Therefore, this review introduces these PCR-mediated techniques independent of culture for viable and dead foodborne pathogen detection from the nucleic acid molecular recognition principal level and describes their whole-process applications in food quality supervision, which provides a useful reference for the development of detection of foodborne pathogens in the future.

Indexed as

dead bacteria detectionDNA-intercalating dyesfoodborne pathogensfood safetypolymerase chain reactionviable bacteria detection

Identifiers

PMID36076861
PMCPMC9455676

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.