ArticleMikrochimica acta2022
Colorimetric assay of phosphate using a multicopper laccase-like nanozyme.
Article in Mikrochimica acta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 37 citations in OpenAlex.
- Rational Design of Mn-APTES/1-Methylimidazole Nanozymes: Enhanced Laccase-like Activity at Near-Neutral pH for Environmental Remediation.International journal of molecular sciences · 2026Article
- Colorimetric Sensors: Methods and Applications.Sensors (Basel, Switzerland) · 2023Article
- Tris-Copper Nanozyme as a Novel Laccase Mimic for the Detection and Degradation of Phenolic Compounds.Sensors (Basel, Switzerland) · 2023Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
A new nanozyme (Cu-NADH) is reported composed of Cu-coordinated nicotinamide adenine dinucleotide (NADH) exhibiting laccase-like activity. The Cu-NADH nanozyme had higher heat tolerance and catalytic efficiency than natural laccase, and its catalytic activity can be enhanced by high concentration of Cl ions and it is intensely inhibited by phosphate. Therefore, a colorimetric method based on Cu-NADH and indigo carmine was successfully developed to detect phosphate in water. This method showed an excellent selectivity for phosphate, and it had a linear relationship in the phosphate concentration range 2-50 μM with a detection limit of 0.37 μM. We believe that this example of coordination between metal ions and biomolecules to mimic natural enzymes can inspire more effective and alternative strategies in nanozyme design and expand their use in sensing and determination.
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