ArticleNature methods2022
Event-triggered STED imaging.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 98 citations in OpenAlex.
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- CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies.Nature communications · 2026Article
- Smart event-triggered MINFLUX microscopy to catch and follow rare events.Nature communications · 2026Article
- Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.Scientific reports · 2026Article
- Review
- A flexible framework for automated STED super-resolution microscopy.Scientific reports · 2026Article
- Smart hybrid microscopy for cell-friendly detection of rare events.Nature communications · 2026Article
- Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis.Journal of ophthalmology · 2026Review
- Advanced Imaging for Live-Cell Spatiotemporal Monitoring: Technologies and Applications.Research (Washington, D.C.) · 2026Review
- Self-Driving Microscopes: AI Meets Super-Resolution Microscopy.Small methods · 2026Review
- Closed-loop optogenetic control of cell biology enables outcome-driven microscopy.Nature communications · 2025Article
- Topology-driven energy transfer networks for upconversion stimulated emission depletion microscopy.Light, science & applications · 2025Article
- Smart 3D super-resolution microscopy reveals the architecture of the RNA scaffold in a nuclear body.Nature communications · 2025Article
- Feature-driven whole-tissue imaging with subcellular resolution.Cell reports methods · 2025Article
- Self-driving microscopy detects the onset of protein aggregation and enables intelligent Brillouin imaging.Nature communications · 2025Article
- Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience.Biosensors · 2025Review
- Imaging of cellular dynamics from a whole organism to subcellular scale with self-driving, multiscale microscopy.Nature methods · 2025Article
- Fast and Long-Term Super-Resolution Imaging of Endoplasmic Reticulum Nano-structural Dynamics in Living Cells Using a Neural Network.Small science · 2025Article
- Task-driven framework using large models for digital pathology.Communications biology · 2024Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monitoring the proteins and lipids that mediate all cellular processes requires imaging methods with increased spatial and temporal resolution. STED (stimulated emission depletion) nanoscopy enables fast imaging of nanoscale structures in living cells but is limited by photobleaching. Here, we present event-triggered STED, an automated multiscale method capable of rapidly initiating two-dimensional (2D) and 3D STED imaging after detecting cellular events such as protein recruitment, vesicle trafficking and second messengers activity using biosensors. STED is applied in the vicinity of detected events to maximize the temporal resolution. We imaged synaptic vesicle dynamics at up to 24 Hz, 40 ms after local calcium activity; endocytosis and exocytosis events at up to 11 Hz, 40 ms after local protein recruitment or pH changes; and the interaction between endosomal vesicles at up to 3 Hz, 70 ms after approaching one another. Event-triggered STED extends the capabilities of live nanoscale imaging, enabling novel biological observations in real time.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.