ArticleHorticulture research2022
Highly efficient CRISPR systems for loss-of-function and gain-of-function research in pear calli.
Article in Horticulture research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Hydrogen sulfide-mediated persulfidation of ERF106 controls pear fruit aroma biosynthesis via disrupting the ERF106-MYB21-FAD8 complex.The Plant journal : for cell and molecular biology · 2026Article
- The Dual Role of Lignin in Fruit Trees: Unraveling Regulatory Networks from Stress Resilience to Quality Control.Plants (Basel, Switzerland) · 2026Review
- Brassinosteroid signaling promotes stone cell lignification by enhancing nuclear accumulation of PbrBEH3 in pear fruit.The Plant journal : for cell and molecular biology · 2026Article
- Agrobacterium rhizogenes-mediated stable and transient transformation systems enable rapid functional validation of C6 aldehyde synthesis genes in peach.BMC plant biology · 2026Article
- ARF6 integrates auxin and gibberellin signaling to promote stone cell lignification in pear via the HB49-MYB169 module.The New phytologist · 2026Article
- The emerging impact of CRISPR and gene editing on global crop improvement.Transgenic research · 2026Review
- Research progress and applications of gene activation editing technology in crops.Frontiers in plant science · 2026Review
- The PbrMADS1-PbrMYB169 complex has uniquely emerged to regulate lignification of stone cells in pear.Journal of integrative plant biology · 2026Article
- Chromatic symphony of fleshy fruits: functions, biosynthesis and metabolic engineering of bioactive compounds.Molecular horticulture · 2025Review
- Highly Efficient Homozygous CRISPR/Cas9 Gene Editing Based on Single-Cell-Originated Somatic Embryogenesis inPlants (Basel, Switzerland) · 2025Article
- Insights into the human cDNA: A descriptive study using library screening in yeast.Journal, genetic engineering & biotechnology · 2024Article
- Article
- Biochemical andFood chemistry. Molecular sciences · 2024Article
- Allelic variation of BBX24 is a dominant determinant controlling red coloration and dwarfism in pear.Plant biotechnology journal · 2024Article
- New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i.Plant methods · 2024Article
- PbrBZR1 interacts with PbrARI2.3 to mediate brassinosteroid-regulated pollen tube growth during self-incompatibility signaling in pear.Plant physiology · 2023Article
- CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.International journal of molecular sciences · 2023Review
- Anthocyanins in Plant Food: Current Status, Genetic Modification, and Future Perspectives.Molecules (Basel, Switzerland) · 2023Review
- Genetic amelioration of fruit and vegetable crops to increase biotic and abiotic stress resistance through CRISPR Genome Editing.Frontiers in plant science · 2023Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas systems have been widely used for genome engineering in many plant species. However, their potentials have remained largely untapped in fruit crops, particularly in pear, due to the high levels of genomic heterozygosity and difficulties in tissue culture and stable transformation. To date, only a few reports on the application of the CRISPR/Cas9 system in pear have been documented, and have shown very low editing efficiency. Here we report a highly efficient CRISPR toolbox for loss-of-function and gain-of-function research in pear. We compared four different CRISPR/Cas9 expression systems for loss-of-function analysis and identified a potent system that showed nearly 100% editing efficiency for multi-site mutagenesis. To expand the targeting scope, we further tested different CRISPR/Cas12a and Cas12b systems in pear for the first time, albeit with low editing efficiency. In addition, we established a CRISPR activation (CRISPRa) system for multiplexed gene activation in pear calli for gain-of-function analysis. Furthermore, we successfully engineered the anthocyanin and lignin biosynthesis pathways using both CRISPR/Cas9 and CRISPRa systems in pear calli. Taking these results together, we have built a highly efficient CRISPR toolbox for genome editing and gene regulation, paving the way for functional genomics studies as well as molecular breeding in pear.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.