ArticlePlant biotechnology journal2022
Multiplexed promoter and gene editing in wheat using a virus-based guide RNA delivery system.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement.Plant cell reports · 2026Review
- Potential unlocked: an atlas of cloned wheat genes for genome engineering and breeding.The New phytologist · 2026Review
- Satellite DNA-targeted CRISPR-Cas9-mediated editing enables chromosome truncation and elimination in wheat.Plant communications · 2026Article
- Wheat's Up with CRISPR-Cas-Current Advances, Obstacles and Perspectives.International journal of molecular sciences · 2026Review
- Plant viruses as next-generation vectors for transgene-free genome editing, gene regulation, and rapid crop improvement.Plant molecular biology · 2026Review
- BSMV-mediated genome editing exhibits host-specific heritability: germline transmission in barley and somatic edits in Nicotiana benthamiana.BMC plant biology · 2026Article
- Virus-Mediated System for Simultaneous Gene Silencing and Genome Editing in Cotton.Plants (Basel, Switzerland) · 2026Article
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Geminivirus-derived replicons: assessment for transient GFP expression in tobacco and tomato.Frontiers in plant science · 2026Article
- Nucleoredoxin 1 in Wheat: Genomic Analysis and Demonstration of Its Role in Redox Homeostasis and Stress Resilience.Plant direct · 2025Article
- Morphogenetic Factors as a Tool for Enhancing Plant Regeneration Capacity During In Vitro Transformation.International journal of molecular sciences · 2025Review
- Integrating genome editing with omics, artificial intelligence, and advanced farming technologies to increase crop productivity.Plant communications · 2025Review
- Virus-induced gene editing free from tissue culture.Nature plants · 2025Review
- Review
- Enhancing virus-mediated genome editing for cultivated tomato through low temperature.Plant cell reports · 2025Article
- Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.The Plant journal : for cell and molecular biology · 2025Article
- Barley stripe mosaic virus-induced gene silencing for functional validation of abiotic stress in barley.Functional & integrative genomics · 2024Review
- Heritable gene editing in tomato through viral delivery of isopentenyl transferase and single-guide RNAs to latent axillary meristematic cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
- Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides.Nature communications · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The low efficiency of genetic transformation and gene editing across diverse cultivars hinder the broad application of CRISPR technology for crop improvement. The development of virus-based methods of CRISPR-Cas system delivery into the plant cells holds great promise to overcome these limitations. Here, we perform direct inoculation of wheat leaves with the barley stripe mosaic virus (BSMV) transcripts to deliver guide RNAs (sgRNA) into the Cas9-expressing wheat. We demonstrate that wheat inoculation with the pool of BSMV-sgRNAs could be used to generate heritable precise deletions in the promoter region of a transcription factor and to perform multiplexed editing of agronomic genes. We transfer the high-expressing locus of Cas9 into adapted spring and winter cultivars by marker-assisted introgression and use of the BSMV-sgRNAs to edit two agronomic genes. A strategy presented in our study could be applied to any adapted cultivar for creating new cis-regulatory diversity or large-scale editing of multiple genes in biological pathways or QTL regions, opening possibilities for the effective engineering of crop genomes, and accelerating gene discovery and trait improvement efforts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.